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  • 發布時間:2019-07-29 22:05 原文鏈接: 常用的βactin引物序列

    human actin f ctc cat cct ggc ctc gct gt 
    human actin r gct gtc acc ttc acc gtt cc 
    product size:268 
    rabbit actin r agt gcg acg tgg aca tcc g 
    rabbit actin f tgg ctc taa cag tcc gcc tag 
    product size:295 
    mouse actin r cgt tga cat ccg taa aga cc 
    mouse actin f aac agt ccg cct aga agc ac 
    product size:281 
    rat actin f TCAGGTCATCACTATCGGCAAT 
    rat actin r AAAGAAAGGGTGTAAAACGCA 
    product size:432 
    human actin r gag cta cga gct gcc tga cg 
    human actin f cct aga agc att tgc ggt gg 
    product size:416 
    mouse actin f tca tca cta ttg gca acg agc 
    mouse actin r aac agt ccg cct aga agc ac 
    product size:399 
    rat actin f CCCATCTATGAGGGTTACGC 
    rat actin r TTTAATGTCACGCACGATTTC 
    product size:150 
    rabbit actin f tct tcc agc cct cct tcc tg 
    rabbit actin r cgt ttc tgc gcc gtt agg t 
    product size:409 


    內參基因名稱 引物 引物最佳退火 擴增 
    基因庫序列號 引物名稱 序列 位置 Tm 溫度C 長度 

    Human actin beta F305 ctgggacgacatggagaaaa 305-324 52.3 
    BC002409 R868 aaggaaggctggaagagtgc 868-849 52.6 59.4 564 
    F1379 agcgagcatcccccaaagtt 1379-1398 57.3 
    R1663 gggcacgaaggctcatcatt 1663-1644 56.3 54 285 

    Rat actin beta F18 cacccgcgagtacaaccttc 18-37 54.5 
    NM_031144 R224 cccatacccaccatcacacc 224-205 54.4 60.4 207 
    F694 gagagggaaatcgtgcgtgac 694-714 54 
    R1146 catctgctggaaggtggaca 1146-1127 53.2 57.1 452 

    Mouse actin beta F91 atatcgctgcgctggtcgtc 91-110 57.5 
    NM_007393 R607 aggatggcgtgagggagagc 607-588 57.8 60.4 517 
    F1566 gtccctcaccctcccaaaag 1566-1585 54.5 
    F1831 gctgcctcaacacctcaaccc 1831-1811 54.4 55.7 266 

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