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  • 發布時間:2022-02-22 10:50 原文鏈接: 環介導等溫擴增技術原理

    環介導等溫擴增(loop-mediated isothermal amplification),是一個比較公認的等溫擴增方法,現如今等溫擴增法很多,比如RCA,NASBA,RPA...如果可以,你可以參考這篇文獻:Loop-mediated isothermal amplification integrated on microfluidic chips for point-of-care quantitative detection of pathogens,它詳述了具體過程,由于它使用的引物為對稱結構,后來已經改進,將引物設計為不對稱結構,從而減少了背景信號的產生,文獻參考:Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology。

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