Rapid solid-phase peptide synthesis using thermal and controlled microwave irradiation
Abstract: A rapid and efficient microwave-assisted solid-phase synthesis method is described for the preparation of thenonapeptideWDTVRISFK, using conventional Fmoc/But orthogonal
protection strategy. The synthesis protocol is based on theuse of
cycles of pulsed microwave irradiation with intermittent cooling of the
reaction during the removal of the Fmoc protectinggroup and during the
coupling. The desired nonapeptide was obtained in highest yield and
purity by employing MicroKantechnology.
The chemical reactions were carried out in a single-mode microwave
reactor, equipped with a fiber-optic probe tomonitor the reaction
temperature continuously.
摘要：本文描述一種快速有效的微波輔助固相合成方法，用以合成序列為WDTVRISFK的短肽，使用傳統的Fmoc（9-芴氧羰基）/tBu（叔丁基）保護基策略。該合成方法是基于反應中的周期性脈沖微波輻射和間歇性冷卻技術，在Fmoc保護基的脫除及偶聯反應中均應用此技術。在應用微反應器技術后得到了高純度和高收率的目標多肽。該反應在一個CEM單模微波反應器中進行，并且使用光纖進行連續測溫。
原文出處：
Rapid solid-phase peptide synthesis using thermal andcontrolled microwave irradiation