GenJet Ver.II，LipoD293及PolyJet針對哺乳動物細胞的DNA體外轉染試劑，均為您提供兩種不同的轉染步驟——一般步驟及高級步驟，這兩種步驟分別針對不同的哺乳動物細胞。高級步驟主要針對難轉的哺乳動物細胞，例如：MDCK，MDA-MB231，Caco-2等細胞。使用一般的轉染步驟若是轉染效率低于5%，那么使用高級轉染步驟則可以將轉染效率提升至60%。
如何選擇轉染步驟則取決于您的細胞特性。對于諸如HEK293，Hela，CHO，3T3，COS，HepG2，LNCaP，PC3，PC12，U2-OS，L929，MCF-7及Huh-7等常用細胞，一般的實驗步驟就能得到滿意的轉染結果。對于諸如MDCK，MDA-MB231，Caco-2，SaoS-2及HUVEC等難轉細胞，可以選用高級轉染步驟。針對您的細胞，若是您尚不明確選擇哪種轉染步驟，我們建議您可先嘗試一般步驟，如果您使用一般步驟得到的轉染效率低于5%，那么您的細胞比較難轉，您可嘗試高級轉染步驟。
Choose a more efficient protocol for your specific cell.
GenJet Ver. II, LipoD293 and PolyJet DNA in vitro transfection reagent all offer two protocols for transfecting mammalian cells--------a general protocol and an advanced protocol. The two protocol are written for transfecting different types of mammalian cells. The advanced protocol which involves a shaving cell technology is for very hard to transfect mammalian cells like MDCK, MDA-MB231, Caco-2, etc. The general protocol usually gives less than 5% efficiency while advanced protocol can get up to 60% efficiency on these problematic cells.
The principle to decide which one protocol is used to your cell is highly dependent upon the nature of your cell. For commonly used cells like HEK293, Hela, CHO, 3T3, COS, HepG2, LNCaP, PC3, PC12, U2-OS, L929, MCF-7 and Huh-7, be sure to use general protocol which usually gives very good transgene expression while the advanced protocol does not help. For very hard to transfect cells like MDCK, MDA-MB231, Caco-2, SaoS-2 and HUVEC, follow the advanced protocol by trypsinizing these adherent cells first followed by incubation of the cell pellet with transfection complex. If you do not know for your specific cells how to choose the more efficient protocol, we suggest you try general protocol first. If you get less than 5% efficiency with the general protocol, then your cell is hard to transfect in nature and you can consider trying advanced one.
