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實驗概要Fix and Perm reagents are designed for use with all commercially available flow cytometers. Alignment and compensation should be performed according to the manufacturer's instructions. Typical staining and scatter patterns are shown below in Figure 1. Clinical Research Applications: Flow cytometric analysis with monoclonal antibodies has historically been restricted primarily to ......閱讀全文
AAA - Amino acid analysis – 氨基酸分析AAS - Atomic absorption spectrometry – 原子吸收光譜AED -&n
AAA - Amino acid analysis – 氨基酸分析AAS - Atomic absorption spectrometry – 原子吸收光譜AED -&n
Take the appropriate set of Fmoc-amino acid stock aliquots for cycle 1 from the freezer, bring to room temperature, and activate by adding DIC (4 μl p
主要試劑1. Blocking reagent after coating: 10% fetal calf serum (FCS) in phosphate-buffered saline (PBS), pH 7.4.2. Cells suspended in complete culture me
Chapter 1: Cell Death - Apoptosis and Necrosis1.1Introduction21.1.1Terminology of cell death21.1.2Differences between necrosis and apoptosis31.1.3Apop
NGS 是一種識別和確認未知致病菌的前景廣闊的技術，然而其在生物防御和公共健康應用等方面的時效性，卻往往因為缺乏快速、有效、可靠的自動DNA樣品制備方法而受到限制。為了突破這種限制，Kim 等設計了一種基于流體分布元件的數字微流體(DMF) 平臺，使得多子系統模塊能夠進入自動NGS庫樣品
DetectionThe choice of the detection scheme depends on several factors: (i) the number of haptens and fluorochromes used for probe labeling; (ii) the
摘要：基于抗體的蛋白質檢測方法，主要有western blot、ELISA、點雜交以及免疫組化等，這些方法被廣泛地應用于科研和診斷領域。在蛋白質的免疫檢測過程中，樣品蛋白首先結合與特異性一抗上，然后再用攜帶標簽（諸如：熒光染料，放射性元素，酶等）的二抗進行檢測。然而，為了避免種間內的交叉反應，必
生物分子發表的代表性文獻 QTRAP：同時具有三重四極桿和線性離子阱性能的獨一無二的LC/MS/MS系統 QTRAP系統最早在ASMS 2002上，作為第一臺商用的線性離子阱發布，是世界上唯一的線性離子阱和三重四極桿的復合
back to topProtocolStandard vs. Rapid Immunodetection ProceduresThere are two types of protocols for immunodetection: Standard and rapid.Standard vs.
實驗概要The Sandwich ELISA measures the amount of antigen between two layers of antibodies (i.e. capture and detection antibody). The antigen
實驗概要We provide a guideline procedure and tips for staining of paraffin embedded sections, including antigen retrieval, chromogenic detecti
DiscussionIn this work we demonstrate that the fast terahertz QWP detector is capable of responding 6.2?GHz modulated terahertz light. We should
離子色譜（IC）發展已經有四分一個世紀，作為一種獨立的色譜分枝，并在許多領域得到廣泛應用。 但離子色譜（IC）與其相似的色譜高效液相色譜（HPLC）相比，柱效要低很多（約為HPLC的1/3），而價格則要高很多（約HPLC的3到5倍）。 提高離子色譜的柱效，是離子色譜研究急需解決的問題，也將是推動離子
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization
實驗概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples contai
2 目前國內外應用于檢測耐藥結核桿菌的分子生物學方法隨著分子生物學的發展，尤其是PCR技術的問世，檢測耐藥可以直接從基因入手，這樣與傳統的結核桿菌藥敏試驗相比，不僅大大縮短了檢測周期，實現了自動化，而且也降低了生物實驗室的危險性。耐藥檢測包括用PCR擴增基因組內攜帶的耐藥性區域，和進行擴增產物的突變
實驗概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples contai
作者：鄧紅艷,張波 作者單位：(成都鐵路中心醫院,四川 成都 610081)【摘要】 目的：評價我院Vitek-32 AMS檢測耐甲氧西林葡萄球菌(MRS)的可靠性。方法：分別采用頭孢西丁紙片擴散法(FOX法)、苯唑西林鹽瓊脂紙片擴散法(OXA
· Protein detection (Aberdeen's Lab)The method used to locate the proteins following 2D-
Materials:Hoechst 33258 (Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500μg/ml (50μl stock + 950μl PBS).7-Amino-actinomycin (
作者：鄧紅艷,張波 作者單位：(成都鐵路中心醫院,四川 成都 610081) 【摘要】 目的：評價我院Vitek-32 AMS檢測耐甲氧西林葡萄球菌(MRS)的可靠性。方法：分別采用頭孢西丁紙片擴散法(FOX法)、苯唑西林
6.2-GHz modulated terahertz light detection using fast terahertz quantum well photodetectorsHua Li,1 Wen-Jian Wan,1 Zhi-Yong Tan,1 Zhan
對于其雷達信號處理器，IWR1642集成了德州儀器（TI）C674x數字信號處理器（DSP）內核（圖4）。 IWR1642 DSP是專為FMCW信號處理而設計的，以600 MHz時鐘運行，并由32 KB L1程序（L1P）和數據（L1d）高速緩存支持，以及256 KB統一程序/數據L2高速緩
隨著高通量測序市場持續火爆，其重要應用無創產前篩查越來越受到市場的追捧。我國監管部門也采用積極的舉措來規范整個市場，今年2月份中國食品藥品監督管理總局、國家衛生計生委聯合發布《關于加強臨床使用基因測序相關產品和技術管理的通知》叫停基因測序臨床應用。然而，僅僅一個月后，國家衛生計生委又發布了《關于開展
實驗概要Capture ELISA (also known as "sandwich" ELISA) is a sensitive assay to quantitate picogram to microgram quantities of substa
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
Gravitational waves from a binary black hole merger observed by LIGO and VirgoNews Release ? September 27, 2017The LIGO Scientific Collaboration and
實驗概要Cytokine ELISPOT has become a powerful routine tool for the analysis of disease- as well as vaccine-induced T-cell responses. The meth