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This procedure describes a convenient method for the isolation of crude nuclear pellets from N. crassa. The method, an adaptation of the one developed by Hautala et al ((1977 J Bact 130:704-713) utilizes a Braun homogenizer to disrupt the cells. The main advantages of the technique are that the cells need not be froen, large amounts of material can be handled, the homogenization is fast and easily controlled, fe......閱讀全文
Adrenal chromaffin granule (chromaffin vesicle) preparationIntroduction. This prep is adapted from the classic paper of Smith and Winkler (Smith
RNA提取（主要內容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc
9. Spontaneous germination. Some genotypes result in spontaneous ascospore germination (for example, the unpigmented ascospores of per-1 Type I). A si
DNA抽提（主要內容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
Botanical Bulletin of Academia Sinica, Vol. 37, 1996The method using NBT as a superoxide radical competitor and a color indicator was also explored to
Assay of superoxide dismutase activity by combining electrophoresis and densitometryAbstract. A modified technique was developed to assay superoxide d
AbstractThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of Helico
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In
AbstractWhen more than one bait will be used to screen a single library, significant time and resources can be saved by performing the interactor hunt
蛋白質提取和純化（主要內容如下）Protein Extraction Protein PurificationProtein PrecipitationColumn PreparatioinQ & A Posted in the Method ForumProtein Extrac
1. Introduction and BackgroundThere is a great need for general methods to characterize the proteins that contemporary biology makes available. The li
實驗概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apopto
Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis.T. Turetsky, E. Aizenman, Y. G
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise 10.3 - Extraction of ChromatinLEVEL IIMaterials Bovine
MN in Human Lymphocytes (method description)Lymphocyte isolation Lymphocytes were isolated using Ficoll-Paque density gradients
實驗概要The E.Z.N.A.? Blood DNA Maxi Kit is designed for isolation of genomic DNA from up to 25 ml of fresh, whole blood treated with any comm
Yeast DNA PreparationYeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (r
實驗概要The E.Z.N.A.? Blood DNA Maxi Kit is designed for isolation of genomic DNA from up to 25 ml of fresh, whole blood treated with any comm
In this section, you will find techniques related to oligonucleotides, such as oligo purification by acrylamide gel, annealing two oligos to make doub
Molecular biology experiments often require preparation of small amounts of DNA from many samples. This abbreviated DNA isolation method yields an ave
Preparation of fungi for DNA extraction typically involves growing cultures in liquid culture in Erlenmeyer flasks, Roux bottles or even microfuge tub
F. Video Microscopy 1. The behavior of migrating cells may be filmed with an inverted microscope fitted with a CCD camera. 2. Determine the
Nonyl Acridine OrangeNonyl acridine orange (A1372) is well retained in the mitochondria of live HeLa cells for up to 10 days, making it a useful probe
Liver is a convenient source for functional intact mitochondria for a number of reasons. Animal tissue is more readily homogenized than plant tissue b
Primary cultures of intrahepatic bile duct epithelial cells isolated and cultured1. Liver was surgically removed and perfused via the hepatic vei
實驗概要The CellTrace? Violet Cell Proliferation Kit provides a versatile and well-retained cell tracing reagent in a convenient and easy-to-u
Preparing Lawn Cells for M13 Cloning (Life Technologies)Lawn cells require the F' episome for M13 infection and may be prepared St
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA