MaterialsSiliconized ultracentrifuge microfuge tubesGTP-depleted microtubules6X SDS loading dye1X SDS loading dyeCoomassie Brilliant Blue R 250 (0.8% in 50% methanol + 10% acetic acid)Destaining solution (13% methanol + 3% acetic acid)Procedure1.Mix buffer and taxol in a siliconized ultracentrifuge microfuge tube, then add motor and GTP-depleted microtubules using a set concentration of microtubules (e.g., ......閱讀全文
DAY 2: Cycling preparation of MT protein.Keep the brains in an evacuated plastic ziplock bag buried in ice from the time of slaughter during transport
生物分子發表的代表性文獻 QTRAP:同時具有三重四極桿和線性離子阱性能的獨一無二的LC/MS/MS系統 QTRAP系統最早在ASMS 2002上,作為第一臺商用的線性離子阱發布,是世界上唯一的線性離子阱和三重四極桿的復合
Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab) Recycling Tubulin (Mitchison Lab) Labeling Tubulin