ExampleTissueBankProtocol1
BackgroundSummarize the purpose and objectives of the tissue bank in this section.2 Eligibility to Participate in the Research Tissue BankThis section should specify who is eligible to participate in the Tissue Bank. If there are plans to collect residual tissue, (for example left over blood samples from a blood draw or......閱讀全文
Example－Tissue－Bank－Protocol1
? ? ? ? ??Background Summarize the purpose and objectives of the tissue bank in this section. 2??????????Eligibility to Participate in the Research
Example－Tissue－Bank－Protocol3
7.2?????????Research with de-identified tissueThis section should describe the process for supplying researchers with tissue samples that will not hav
Example－Tissue－Bank－Protocol2
5.1?????????Record and Tissue Sample HandlingFor tissue samples arriving with identifiers, this section must?clearly?state whether any link is being m
Tissue－Harvest－Protocol
TISSUES TO BE PROCURED(minimally and preferably within 5-8 hours after death):1. Brain2. Liver3. Muscle4. Skin5: Others as the specific case dictatesP
Tissue－preparation－protocol－for－ChIP
實驗概要This protocol ?describes how chromatin is prepared from tissue, which can subsequently ?be used for chromatin immunoprecipitation (ChIP). It is re
HP－Tissue－DNA－Maxi－Protocol
實驗概要The E.Z.N.A.? ?HP Tissue DNA Maxi Kit is designed for efficient recovery of genomic ?DNA up to 60 kb in size from up to 2 grams of tissue samples.
HP－Tissue－DNA－Midi－Protocol
實驗概要The E.Z.N.A.? HP Tissue DNA Midi Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from up to 500mg of tissue samples.
Vacuum/Spin－Protocol－for－Tissue－DNA－Extraction
實驗概要The E.Z.N.A.? ?Tissue DNA Kit provides a rapid and easy method for the isolation of ?genomic DNA for consistent PCR and Southern analysis. Up to 3
E.Z.N.A.?－Protocol－for－Tissue
實驗概要The E.Z.N.A.? ?Tissue DNA Kit provides a rapid and easy method for the isolation of ?genomic DNA for consistent PCR and Southern analysis. Up to 3
E.Z.N.A.?－Protocol－for－ParaffinEmbedded－Tissue
實驗概要The E.Z.N.A.? Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to
Master－Cell－Bank
PurposeTo describe the preparation of a Master Cell BankSafetySee SP 09-001 for lab safety considerations for the cell culture lab.EquipmentLaminar Fl
Working－Cell－Bank
1) Keep prepared solutions on ice.2) Determine total cell count of cells to be frozen. (e.g. 1 X 108 )3) Determine number of vials to be frozen. (e.g.
Protocols－for－LCM－preparation－and－analysis
Protocols for LCM preparation and analysis?I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA.?EmbeddingB.?CuttingC.?StainingII. Pr
E.－Immunohistochemi...
實驗概要We provide a ?guideline procedure and tips for staining of paraffin embedded sections, ?including antigen retrieval, chromogenic detection and flu
PCR－from－Tissue
collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH put in boiling H2?O for 30 sec (optimu
Tissue－Culture－Media
We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in
PCR－from－Tissue
collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOHput in boiling H2O for 30 sec (optimum may ne
TISSUE－CULTURE－ON－COVERSLIPS
I.?Purpose:A. Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected
PCR－from－Tissue
1.collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH 2.put in boiling H2O for 30 sec (optimum
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mo
Multicolour－3DFISH－in－vertebrate－cells5
Author Notes After the fourth round of DOP amplification the probe quality is considerably reduced. Use the low stringency cycles only in case
轉基因
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline?(University of Michigan Transgenic Animal Model Core)Thi
反向PCR
主要內容如下：·?????????RT-PCR·?????????Competitive and Quantative RT-PCR·?????????In Situ RT-PCR·?????????RL-PCR·?????????DNA Contamination·?????????RT-PCR
PCR－from－Plant－Tissue
1.protocol (1)collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH (2)put in boiling H2Ofor 30
Dissociation－of－spleen－and－hemopoietic－tissue
You need to buy glass slides with frosted, sandblasted ends (Fisher Scientific, Catalog N.? 12-552). Frosting by painting (e.g.Superfrost) should?not
PCR－from－Plant－Tissue
PCR from Tissue?Reference:??Klimyuk et.al., 1993, Plant J. 3:493-494?Last updated: 1/27/00?By: Kay Schneitz?? ? ???collect piece of tissue (e.g., piec
Stock－solutions－for－tissue－culture
The kitchen makes Tris, TD, Tryp/TD, PBS, and VE.Tris?is a fairly complex, Tris-buffered physiological saline solution. It is used to wash cells and i
Cell－and－tissue－lysis－hub
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison
DNA－Extraction－from－Tissue
實驗概要DNA extraction from tissue.主要試劑Extraction buffer100 mM Tris-HCl (pH 8.0)?????100 mM EDTA (pH 8.0)?100 mM Na-Phosphate (pH 8.0)???1.5 M NaCl1% CTAB
碳水化合物分析
Carbohydrate Assay?(Hancock Laboratory) (Accessible only by IE) This protocol is used to determine the relative amounts of LPS CHO present in a giv