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An actively growing culture of cells is required, i e 2 - 3 d ES cell culture. The total number of cells needs to be between 106 - 107 cells.N B Read notes at end of method.1) Harvest cells in the usual manner, into a conical bottom centrifuge tube.2) Centrifuge to remove medium.3) Resuspend cell button by flicking the bottom of the tube.4) Add 8 ml of warmed KC1 solution to each tube and mix gently with a pipette.5)......閱讀全文
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routin
MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURERoutine Culturing of ES CellsISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTSMITOMYCIN C TREATMEN