WIKI資訊
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-AAD, e.g., Calbiochem, CA)3. Human AB serum, heat-inactivated (HAB, e.g., Irvine Scientific, CA)4. Refrigerated centrifugePreparation of 7-AAD stock solutionDissolve 7-AAD powder (1mg) first in 50 microliters of absolute methanol, then add 950 microliters of 1 X PBS. Fi......閱讀全文
實驗概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apopto