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based on splitting onto on plateWarm collagenase media to 37°C in a water bath.Aspirate media off of cell culture plate.Add the following amount of collagenase0.5ml/well of 4 well plate1.0ml /well of 6 well plateIncubate at 37 °C for 5-10 minutes; stop incubation when edges of colonies begin to pull away from the plate.Aspirate the collagenase and add appropriate volume (3ml) of conditioned media (CM) to the plate (s......閱讀全文
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
Flow cytometry analysis of the quantitative expression of 3 key pluripotency-associated transcription factors (Nanog, OCT3/4 and SOX2) complemen
Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis.T. Turetsky, E. Aizenman, Y. G
結論· 我們在C1TM單細胞自動制備系統開發了一種簡潔的實驗方案，能以最少的手工操作，在不到24小時內，平行處理高達96個單細胞，對其miRNA表達譜進行分析。· C1 miRNA STA實驗方案使用了Life Technologies為miRNA優化過的試劑。特別的，Meg
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
Introduction MicroRNA (miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate gene expression by both disrupting mess
實驗概要The protocols in this section describe the steps involved in differentiating neural stem cells (NSC) to neurons, astrocytes, and oligo
Noninvasive Human Nuclear Transfer with Embryonic Stem CellsSohyun L. McElroy1 and Renee A. Reijo PeraCenter for Human Embryonic Stem Cell R
實驗概要Directed differentiation of specific lineages has been a focal point in the field of human embryonic stem cell (hESC) research. Cell r
IntroductionHuman embryonic stem (hES) cells are pluripotent stem cells derived from pre-implantation embryos that can be maintained and expanded in a
實驗概要stem cell culture protocol主要試劑cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
Isolation of AE Cells1. Human placentae were obtained with the approval of the institutional review board, after uncomplicated elective caesa
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse or Human-Conditioned Med
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
干細胞是一種能夠長期存活，且具有不斷自我繁殖能力和多向化潛能，幾乎存在于所有組織中的原始細胞。近年來隨著科學家們研究的深入，干細胞在血液系統疾病、神經系統疾病、心血管疾病、自身免疫系統疾病以及內分泌疾病等各種疾病的治療上讓人們看到了希望。 干細胞技術是當今醫學研究最前沿也是最熱門的方向之一，近
干細胞培養制造技術新進展！ 干細胞是一種能夠長期存活，且具有不斷自我繁殖能力和多向化潛能，幾乎存在于所有組織中的原始細胞。近年來隨著科學家們研究的深入，干細胞在血液系統疾病、神經系統疾病、心血管疾病、自身免疫系統疾病以及內分泌疾病等各種疾病的治療上讓人們看到了希望。 干細胞技術是
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routin
Ready-to-use Eppendorf CCCadvanced? FN1 Motifs Surface for Xeno-Free Expansionof Human Pluripotent Stem CellsAurélie Tacheny1, Silvia Tejerina1, Wiame
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho
關鍵詞：人類干細胞來源的心肌細胞 Human stem cell-derived cardiomyocytes (hSC-CMs) 搏動頻率 beat frequency; 場電位時程 field potential duration (FPD)慢性藥物作用 chronotropic drug ef
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
“We picked 42 genes to validate on the BioMark system,” Dr. Yao said. “We picked them to represent different functional categories.”“We used the F
基因敲除可以說是基因組 學、細胞分離培養以及轉基因技術的組合。那么基因敲除的原理是什么呢？ 基因敲除的方法有哪些呢？在此，做個小結，以供大家學習。一.概述：基因敲除是自80年代末以來發展起來的一種新型分子 生物學技術，是通過一定的途徑使機體特定的基因失活或缺失的技術。通常意義上的基因敲除主要是應用D
實驗概要Human embryonic stem cells can be differentiated into neural-, mesenchymal and hematopoetic stem cells. This product is intended for t
Materials and MethodsCell culture conditions and surface transitionCryopreserved hiPSCs (SC102A-1, SBI?, USA) were initially thawed and pre-cultivat
Single-embryo Gene Expression for Early Embryo DevelopmentMylene Yao, M.D. Assistant ProfessorDept. of Obstetrics and Gynecology Stanford UniversityMy
《Cell Stem Cell》雜志是2007年Cell出版社新增兩名新成員之一（另外一個雜志是Cell Host & Microbe），這一雜志內容涵蓋了從最基本的細胞和發育機制到醫療軟件臨床應用等整個干細胞生物學研究內容。這一雜志特別關注胚胎干細胞、組織特異性和癌癥干細胞的最新成果。
Stem cell di?erentiation assaysAdMSCs were harvested from both shake and spinner ?asks into 50 ml tubes. Once the microcarrier beads settled to the
生物分子發表的代表性文獻 QTRAP：同時具有三重四極桿和線性離子阱性能的獨一無二的LC/MS/MS系統 QTRAP系統最早在ASMS 2002上，作為第一臺商用的線性離子阱發布，是世界上唯一的線性離子阱和三重四極桿的復合