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  • 發布時間:2020-07-21 10:35 原文鏈接: 45種培養基配方(細菌培養基與植物培養基)(五)


      37. Clostridium Pasreurianum Synthetic Medium (巴氏梭狀芽孢桿菌合成培養基)
      Glucose (葡萄糖) 1% MnSO4 0.001%
      KH2PO4 0.05% FeSO4 0.001%
      K2HPO4 0.05% Yeast extract(酵母膏) 0.1%
      MgSO4.7H2O 0.02% Peptone (蛋白胨) 0.01%
      NaCl 0.001% CaCO3 0.5%
      Distille water (蒸餾水) 100% pH 7.0

    38. Yeast Extract-Medium Extract Agar Ⅱ (酵母膏、麥芽膏瓊脂 Ⅱ)
      Yeast extract (酵母膏) 4g Mall extract (麥芽膏) 10g
      Glucose (葡萄糖) 4g Agar (瓊脂) 17g
      Distilled water (蒸餾) 1000ml pH 7.3 


      39. Oatemeal Agar (燕麥粉瓊脂)
      Oatemeal(infusion)[燕麥粉浸液] 20g Agar (瓊脂) 18%
      Trace element solution (跡量鹽溶液)1ml
      Distilled water up to (用蒸餾水補充至1000毫升) 1000ml pH 7.2
      [Note]:Trace element solution (跡量鹽溶液):
      FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g 


      ZnSO4.7H2O 0.1g Distilled water (蒸餾水) 100ml
      40. Calcium Malate Agar (蘋果酸鈣瓊脂)
      Calium malate (蘋果酸鈣) 10g K2HPO4 0.5g
      NH4Cl 0.5g Glycerol (甘油) 10g
      Agar (瓊脂) 15g Distilled water (蒸餾水) 1000ml 


      41. Thiobacillus Ferrooxidans Medium (氧化亞鐵硫桿菌培養基)
      (NH4)2SO4 0.15g KH2PO40.05g
      KCl 0.05g MgSO4.7H2O 0.5g
      Ca(NO3)2.4H2O 0.01g FeSO4.7H2O 50g
      Distilled water (蒸餾水) 1000ml pH 2.0 


      42. Thiobacillus Thiooxidans Medium (氧化硫硫桿菌培養基)
      (NH4)2SO4 0.3g KH2PO4 3-4g
      CaCl2 (anhydrous) [無水] 0.25g MgSO4.7H2O 0.5g
      FeSO4.7H2O 0.001g Distilled water (蒸餾水) 1000ml
      pH 3.5-4 


      43. Plasma Substitute Medium (代血漿培養基)
      Sucrose (蔗糖) 12.5-15% Peptone (蛋白胨) 0.5%
      KH2PO4 0.03% Na2HPO4.12H2O 0.14%
      pH 7.0-7.2


      44. Skim Milk Medium (脫脂牛奶培養基) ATCC 183
      脫脂牛奶 100g 去離子水 1000ml
      適用范圍:保加利亞乳桿菌、嗜熱乳酸鏈球菌 


      45. Pea Agar (豌豆瓊脂)
      Glucose (葡萄糖) 1% Peptone (蛋白胨) 0.5%
      NaCl 0.5% Agar (瓊脂) 2.0%
      Pea infusion (counted by NH2) [豌豆浸汁(以NH2計)] 12-14 mg∕100
      pH (before sterilization) [消前] 7.2-7.5
      [Note]: Preparetion of pea infusion (豌豆浸汁的制法) :
      Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water.
      Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for
      antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge
      in order to avoid that the stopper fall off due to gas pressure. Place the
      bottle at 37℃ for 7-10 days. Filter the infusion, then boil the filtrate to
      escape the chloroform. Filter again Distribution and autoclave at 120℃ for 30
      minutes. Store at cool contition .
      (稱取白豌豆900克,置于5公升廣口瓶中,加自來水3公升,用硫酸調pH至4.3-4.5,再加氯仿30毫升(防腐),加膠塞并用紗布扎口(防止沖脫),放37℃恒溫室7-10天。取出用濾紙過濾,濾液煮沸20分鐘,以除氯仿,并再次以濾紙過濾,除沉淀。濾液分裝,于120℃30分鐘滅菌。后冷藏備用。)

      The standard quality of pea infusion as following (豌豆浸汁質量標準):
      Aminonitrogen(氨基酸): 160-200 mg∕100ml
      Soluble phosphoruse(溶磷): 400-600 μg∕ml
      Color(色澤): auratus,transparent (金黃、透明)


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