WIKI資訊
Freezing ESCs 25. Passage the ESCs as described in Step 22. Resuspend the cells in 3 mL of freezing medium for OP9 cells. 26. Aliquot 1 mL of cell suspension per cryovial (~3-6 x 105 cells/cryovial). 27. Freeze the cells at -80°C, and then transfer them to liquid nitrogen for storage. Establishing ESC/OP9-DL1 Cell Co-culturesDay 0: Initiation of Co-culture S......閱讀全文
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl