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Roche公司的RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes下載網址：http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/no1_03/PDF/p22_23.pdf還有一份protocolhttp://www.ualberta.ca/PERINATAL/facilities/pdf/RNase%20Protection%20Assay.pdf可能出現的問題及應對措施1.No Discrete Bands, Only Smears－－RNA degraded, or the the RNase Digestion was too harsh.Try reducing the concentration of RNase A to 1 mg/ml or digest for a shorter period......閱讀全文
Polyacrylamide Gel Electrophoresis (PAGE)for use withRibonuclease Protection Assay (RPA):1. Making the Gel: 5% Denaturing gel for Ribonuclease P
Cytokine BioassaysIntroductionBiological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary cel
The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by th
· RNA Gel (Crawford Lab)· Gel Electroph
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IQuantizatio
實驗概要The PureLink? Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et al. 1999), we have systematically analyzed t
Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not qu
The siRNA user guide (revised May 6, 2004) Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysa