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Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem CellsThe dissociation of adherent mesenchymal stem cell (MSC) monolayers with trypsin and enzyme-free dissociation buffer was compared. A significantly lower proportion of viable cells were obtained with enzyme-free dissociation buffers compared to trypsin. Subsequently, the dissociated cells were re-seeded on new ......閱讀全文
Flow Cytometric AnalysisPrior to flow cytometric analysis, the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) was added to cell suspensio
演示了采用Young-Laplace方程擬合時各家接觸角儀公司軟件的區別。從測試結果來看，接觸角變化范圍從103－105度之間，屬于一個合理的變化范圍。這種變化由各種因素造成，如水的揮發等。另可參考如下資料，我們發現國外的部分生產廠的接觸角儀軟件使用Young-Laplace擬合時，90－140度時
1. Name：Tuning structure and properties of ultra-low cross-linked temperature-sensitive microgels at interfaces via the adsorpti
An Introduction to BiocomputingVersion 2.01October 1996David Steffen, Ph.D.President, Biomedical Computing, Inc.6626 WestchesterHouston, Texas 77
The onion root tip and the whitefish blastula remain as the standard introduction to the study of mitosis. The onion has easily observable chromosomes
Generation of a Light Curve To address the hypothesis concerning photosynthetic efficiency it is necessary to expose sun and shade leaves to a ran
Sapphire Window Development and Testing.The effort of this work was devoted to fabricating ultrathin strengthened sapphire disks and to accurately
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epi
Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the c
Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to h
Slide Coating13. Just before use, dilute the poly-D-lysine stock solution 1:50 in H2O. Add 0.2 mL of diluted poly-D-lysine to each well of a
Comparison of normalisation methodsThere is an ongoing debate what is the best way to normalise qPCR data. Reference genes are the most common method,
Comparison of normalisation methodsThere is an ongoing debate what is the best way to normalise qPCR data. Reference genes are the most common me
Image(Lighting)Contrast The ratio of difference between light and dark as shown on a screen. The change of contrast level refers to &
實驗概要We provide a guideline procedure and tips for staining of paraffin embedded sections, including antigen retrieval, chromogenic detecti
Purification of PCR Products in Preparation for CloningJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid
Figure 4. Comparison of different decoy strategies. The estimated FDR of each decoy method is compared with the real FDR. “Decoy: + 11
Thermo Fisher 'Going Directly After' $250M Q-TOF Market with Release of New Q Exactive Mass SpecDENVER — With the release of its new Q Exactive mass s
2、實驗步驟1在微孔板的每一微孔中加入25ul 0.5M的NaOH溶液。2將75ul標準品、質控品和血斑樣品洗提液分別加入到相應的微孔中,稍振板以便混合均勻3在每一微孔中加入100ul臨時配置好的酶溶液4室溫（18-25℃）下溫育30分鐘5在每一微孔中加入100ul底物溶液，振蕩器（300-500r
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
John “JC” Carver, a payload integration engineer with NASA Kennedy Space Center’s Test and Operations Support Contract, uses a FluorPen to measure
Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
A few cell types are thin enough to be viewed directly in a microscope (algae, protozoa, blood, tissue cultures), but most tissues (kidney, liver, bra
實驗概要This protocol describes steps for fluorescent in situ hybridization (FISH) to Drosophila embryos using Tyramide Signal Amplification (
Identify reuses of GEO datasetsAimThe aim of this protocol is to collect data on the reuses of datasets in the published literature. This particular p
Preparation of Glutathione-S-Transferase (GST) Fusion ProteinsMargret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphia,
2013年5月16日-19日，由中國化學會主辦、廈門大學承辦、復旦大學、浙江大學協辦的為期四天的第八屆全國微全分析系統學術會議、第三屆全國微納尺度生物分離分析學術會議暨第五屆國際微化學與微系統學術會議在美麗的海濱城市廈門隆重召開。以下是微全分析專場報告。上海交通大學
Determination of Accuracy of the Competitive PCRTo test the precision of the results obtained with this competitive PCR, five different amounts of T (