WIKI資訊
INTRODUCTIONThe Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells) must be competent to generate many thousands of differentiated neurons through asymmetric divisions during normal development. Studies in fly neuroblasts have been instrumental in identifying how the establishment and maintenanc......閱讀全文
實驗概要The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells)
實驗概要A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic b
EuthanasiaEuthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress to th
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization
實驗概要A method of identifying and enumerating specific cell types in a heterogeneous population of cells by enhancing the specific staining
CD7Membrane glycoprotein and Fc receptor for IgM Homologous to TCR gamma, Ig kappaMembrane expression early during T ontogeny, before TCR rearran
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
實驗概要Dopaminergic (DA) neurons are located in the ventral midbrain (VM). The ability to isolate precursor cells and neurons from the VM pro
A few cell types are thin enough to be viewed directly in a microscope (algae, protozoa, blood, tissue cultures), but most tissues (kidney, liver, bra
實驗概要A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysi
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF&#
Introduction: This method for the detection of cellular proliferation includes several modifications of a previously published protocol (Hayashi, et a
INTRODUCTIONThe formidable size and structure of polytene chromosomes allowmapping of chromosomal protein distributions at very high resolution.This p
實驗概要The ability to culture primary neurons under serum-free conditions facilitates tighter control of neuronal studies. Some serum-free me
一、非特異性染色的主要因素 組織的非特異性染色的機理很復雜，其產生的原因主要可分為以下幾點： （1）一部分熒光素未與蛋白質結合，形成了聚合物和衍化物，而不能被透析除去。 （2）抗體以外的血清蛋白與熒光素結合形成熒光素脲蛋白，可與組織成分結合。 （3）除去檢查的
四、熒光圖像的記錄方法 熒光顯微鏡所看到的熒光圖像，一是具有形態學特征，二是具有熒光的顏色和亮度，在判斷結果時，必須將二者結合起來綜合判斷。結果記錄根據主觀指標，即憑工作者目力觀察。作為一般定性觀察，基本上可靠的。隨著技術科學的發展，在不同程度上采用客觀指標記錄判斷結果，如用細胞分光光度計，圖像
The Effects of NiCl2on Spicule FormationJessica Ann Billet, Franklin and Marshall, Class of 2000Background and ObjectiveSea urchins exhibit radial hol
實驗概要This method is use to extract short RNAs from plant tissue. Some of the variables (e.g. centrifugation speeds×, precipitation
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
Suitable for use on single-cell suspensions from peripheral blood, lymphoid tissue or cultured cell-lines.Sample Preparation and FixationHarvest cells
12. Hold the salivary glands at the common duct with tweezers.Transfer the glands to a drop of fixing solution on a siliconizedcoverslip.&nb
3. Methods3.1 RNA Probe Preparation1. Different strategies can be used to prepare template DNA for synthesizing antisense RNA p
PurposeThis experiment will study effects of lithium chloride on sea urchin development, forcusing on archenteron formation.IntroductionGastrulation i
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
Abstract Choriallantoic membrane (CAM) limb grafting functions as a method to isolate the inductive eve
OverviewR&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been devel
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous SystemScott R. Hutton and Larysa H. Pevny1UNC Neuroscie
Hematoxylin and Eosin Staining of Tissue for LCM (Arcturus) Immunohistochemical Staining (IHC) (Arcturus)For optimal LCM fr
實驗概要The following is a general procedure guide for preparation and staining of formalin-fixed, paraffin-embedded tissues using a purified,
E.M. PROCESSING SCHEDULE - EPOXY RESINFix tissue in 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer at 4oC, for a minimum of 4 hours. Tissue shou