ChangeinHemoglobinLevelsduetoAnesthesiainMice3
The mean hemoglobin in group 1 was 9.9 mmol/L prior to the anesthesia and decreased to 8.7 mmol/L 10 min after administration of the anesthesia (p < 0.001). At t = 20, 30, and 45 min, it remained at approximately 8.9 but decreased further and was 7.9 mmol/L at termination of the study. Group 2, which received no anesthesia, remained at the same level of hemoglobin (mean 9.5 mmol/......閱讀全文
Change－in－Hemoglobin－Levels－due－to－Anesthesia－in－Mice1
An Important Confounder in Studies on Hematopoietic Drugs Analgesic and anesthetic drugs may have an impact on the results achieved from animal expe
Change－in－Hemoglobin－Levels－due－to－Anesthesia－in－Mice2
Table?1?The design of the cross-over study Blood sample Number (days) Group A (n?=?6) Group B (n?=?6) 1 (0) + anesthesia
Change－in－Hemoglobin－Levels－due－to－Anesthesia－in－Mice3
The mean hemoglobin in group 1 was 9.9?mmol/L prior to the anesthesia and decreased to 8.7?mmol/L 10?min after administration of the anesthesia (p?
Guidelines－for－Retroorbital－Bleeding－in－Laboratory－Rats－and－Mice
Materials Needed:Micro pipettes (l00 μl) or Pasteur pipettes drawn to fine tip2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samp
無創血壓計應用論文：動物用血壓計（三）
TABLE IPrimer sequences for mRNA analyses Sequences are listed 5' to 3'.Quantitative Real-time PCR by SYBR Green Detection Method-The mRNA l
Blood－collection－and－administration－of－fluids－and－drugs－(mouse)2
Intraperitoneal:Equipment:?Syringe and 23 to 27 g, 1/2 to 1-inch needle, preferably with a short bevel.Volume:?The volume injected IP into an adult mo
The－Effect－of－pH－Levels－By－Varying－the－Acidity－of－theExtracellular－Solution
BackgroundZebrafish,?Brachydanio rerio, have a distinct embryonic development. The embryo goes through a process?called epiboly. Epiboly, as defined i
Invivo－evaluation－ofdrug－lead－candidates－by－intravenous－continuous－infusion
In vivo?evaluation of drug lead candidates by intravenous continuous infusion Sang Ho Lee Wesley Shoop Bruce Michael Thomas Felcetto Sheo Singh
Role－of－PPARgamma－Coactivators－in－Obesity－and－Thermogenesis
Nuclear hormone receptors are transcription factors that bind DNA and regulate transcription in a ligand-dependent manner. PPAR-gamma is a member of t
MicroRNA－Expression－Profiling－by－Bead－Array－4
Bead-array-based microRNA detection technology, including the bio-statistic analysis, is currently not well established or widely used and we have app
Tamoxifen－administration－to－mice
One of the uses of?tamoxifen?in the lab is the activation of enzymes deactivated via an estrogen-binding domain, like Cre-ERT?(Cre-LBD). Tamoxifen is
mice－islet－isolation
1.?Islets of Langerhans were isolated from 5- to 7-week-old nonobese diabetic (NOD) mice. 2.?Which involves cannulation of the common bile duct and
Hemoglobins－Chaperone
The function of hemoglobin in oxygen transport by red blood cells requires precise assembly of a tetramer of two beta-subunits and two alpha-units. If
Polyphenoloxidase－(catechol－oxidases)－assay
Browning of the cut surface of some fruits and vegetables is due the presence of a group of enzymes called polyphenoloxidases. These enzymes are relea
In－Vivo－Luciferin－Imaging－Procedure
Mice are injected by an intraperitoneal route with a Luciferin solution (15 mg/mL or 30 mg/kg, in PBS, dose of 150 mg/kg) that is allowed to distribut
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia??Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are?analgesia?(pain relief),?amn
全基因組分析發現多個疾病相關基因
來自倫敦帝國理工學院的研究人員通過多次全組關聯，發現了多個與疾病相關的基因，其中包括了糖尿病，冠心病等現代社會熱點關注的疾病，這些成果陸續發表在《Nature Genetics》，《JAMA》等雜志上。 第一篇文章中，研究人員找到了控制電信號，也就是控制心律的基因，這種被稱為SCN10A的基因受
Osteoblast－Harvest(Adult－Mice)
MaterialInstrumentsSolutions1 Curve Scissor50 ml tube 70% Etoh1? Straight ScissorEtoh Spray bottle1 Bone Scraper1 bottle of PBS2 Forceps (1 medium, 1
Adiponectin－Replenishment－Ameliorates－ObesityRelated－Hypertension（四）
Discussion??? The major findings of the present study were the following: (1) adiponectin supplementation reduced the SBP of spontaneously hyperte
全基因組分析發現多個疾病相關基因
來自倫敦帝國理工學院的研究人員通過多次全基因組關聯分析，發現了多個與疾病相關的基因，其中包括了糖尿病，冠心病等現代社會熱點關注的疾病，這些成果陸續發表在《Nature Genetics》，《JAMA》等雜志上。 第一篇文章中，研究人員找到了控制電信號，也就是控制心律的基因，這種被稱為SCN
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal4
Other?Analgesics Analgesics are pain relievers most often given after a surgery. Narcotic analgesics have already been described above. Nonsteroidal
Harvesting－Hematopoietic－Cells－from－Mice
Materials 4 mice from each genotype 4 Ly5 mice Buckets with wet ice 3x Bucket with dry ice 1x Dewar flask with liquid nitrogen 10
Isolation－of－kidney－glomeruli－from－mice
Isolation of mice glomeruli1.?Mice were anesthetized by an intraperitoneal injection of Avertin (2,2,2-tribromoethyl and tertiary amyl alcohol; 17
活體成像皮下成瘤實驗操作方法
Materials:MDA-MB-231-Luc and HCT116-Luc cellsMice: 2 subcutaneous HCT116-Luc tumor-bearing female BALB/c nude mice;4 na?ve female BALB/c nude mice;Art
無創血壓計應用論文：動物用血壓計（四）
Ang II-stimulated Cardiac Dysfunction in ARKO Male Mice-We also evaluated the cardiac performance of these mice using echocardiography. Although l
Regulation－And－Function－Of－ChREBP－in－Liver
Liver is the major site for carbohydrate metabolism (glycolysis and glycogen synthesis) and triglyceride synthesis (lipogenesis). While insulin was lo
Adiponectin－Replenishment－Ameliorates－ObesityRelated－Hypertension（一）
Adiponectin Replenishment Ameliorates Obesity-Related Hypertension作者：Koji Ohashi; Shinji Kihara; Noriyuki Ouchi; Masahiro Kumada; Koichi Fujita; Aki
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（八）
In summary, we suggested that “acute” response could elicit innate and Th1 adaptive immunity, reversing the tumor-mediated immunosuppressive envi
Avertin－(2,－2,－2Tribromoethanol)
This is prepared as a 1.2% solution and used in mice at a dosage rate of 0.2 ml/10grams body weight which approximates 240 mg/kg. This compound is sub
血紅蛋白電泳（hemoglobin－electrophoresis）
實驗原理血紅蛋白電泳（hemoglobin electrophoresis）目的是檢出和確認各種正常和異常的血紅蛋白。根據不同的血紅蛋白帶有不同的電荷，等電點不同，在一定的pH緩沖液中，血紅蛋白的等電點小于緩沖液的pH時帶負電荷，電泳時在電場中向陽極泳動，反之，Hb帶正電荷向陰極泳動。在一定電壓下，