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Purpose:Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40 glycerol. It is lab policy to prepare a frozen stock of newly acquired or created strains for the archives as soon as possible.Time required:Cells are grown 6 hours to overnight; a total of less than 5 minutes bench time for each strain.Procedure:Day 1Inoculate a 15 ml culture tube containing 5 ml o......閱讀全文
Preparing Overnight Bacteria Culture (LaboratoryExperiments.com)This is a basic procedure for high school students and useful for those who are n