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1) After ECL development, wash membrane once for 10min with PBST.2) Incubate the membrane in stripping buffer (see below) in a heat-sealed plastic bag for 30min at 50oC with occasional mixing. For low-abundance antigens, the strip can be done overnight at RT with constant agitation.3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Strippi......閱讀全文
Western 雜交（主要內容如下）Preparing of Protein LysatesWestern BlottingFar Western BlottingSemi Dry BlottingStripping MembranesTrouble Shooting and Others
back to topProtocolStandard vs. Rapid Immunodetection ProceduresThere are two types of protocols for immunodetection: Standard and rapid.Standard vs.
實驗概要The following protocol provides a method of removal of antibodies from western blots.實驗原理Stripping is the term used to describe the removal
分析測試百科網訊 因新型冠狀病毒肺炎疫情防控工作需要，陜西省疾病預防控制中心需緊急采購應急設備一批，包括PCR儀，雙色紅外激光成像儀（進口），二氧化碳培養箱（氣套紅外），溫度壓力檢測儀，脈動真空高壓滅菌器，制冷/加熱循環水浴（進口）等。圖片來源于網絡 一、項目名稱： 項目名稱：應急設備 需
1. Optional: "Renature" gel- this is thought to permit some refolding of proteins and may be important in finding epitope recognition of mon
co-IP assays can be performed between endogenous proteins or transiently or stably expressed exogenous - usually tagged - proteins. The advantage to u