Bruker推出新一代超高靈敏度離子阱質譜amaZon

布魯克推出新一代超高靈敏度離子阱質譜amaZon 重新定義離子阱質譜性能

新型的amaZon是第一個雙離子漏斗的離子阱質譜，提高10倍靈敏度，并使用第二代ETD技術用于蛋白質組學，質量分辨率高達20,000，最高的采集速度

　　2009年5月11日

　　布魯克.道爾頓公司今天發布新一代超高靈敏度的amaZon離子阱系列質譜。比現有平臺提高了10倍的MS/MS靈敏度，新型的技術可以加速數據采集速度，顯著提高質量分辨率，第二代快速靈敏的ETD/PTR 能力，成為最高性能的離子阱，可獲得無與倫比的數據質量，和應用于廣泛范圍的超強的靈活性。

　　amaZon建立在Bruker公司長期的離子阱技術之上，提高了在以下研究領域的能力：從下至上(bottom-up)的蛋白質組學、高通量和超高靈敏度翻譯后修飾分析、代謝組學、化學物篩選及鑒定，化學和結構的精細分析。amaZon系列現在有兩個版本：用于蛋白質組學的amaZon ETD，和用于小分子等應用領域的amaZon X。

　　新型amaZon包含下列無可匹敵的性能：

      第一個商用的雙離子漏斗傳輸的離子阱，把靈敏度提高到一個新臺階

      無與倫比的高達52,000 u/秒的掃描能力，和更高的分辨率，適用于UPLC應用，并在數據依賴(data-dependent )的MS/MS和MSⁿ實驗中具有最快的duty cycle(譜圖采集時間)

      在50~3000 amu全掃描模式下可獲得20,000的分辨率：合并了BrukerZL的峰識別(peak recognition)技術SNAP II?后，amaZon甚至對于ETD/PTR碎片譜中帶多電荷的離子也能提供同位素峰的分辨，可高確定地測定它們的單同位素質量

      最新的ETD/PTR技術提供了最有效的、特別穩定和靈敏的多肽和蛋白碎片，以及高通量的翻譯后修飾分析。建立在Bruker公司最先商業化的、成功的ETD技術基礎上，合并了從下至上的CID或ETD譜，以及從上到下的ETD/PTR譜這些信息，可以建立蛋白精細表征的新的方法學

      零延遲的高速離子極性切換，而不降低靈敏度，不增加時間。在兩種極性下均可獲得高達20 Hz的譜圖采集速度，是同UHPLC超快速液相分離聯用的理想選擇。amaZon還支持MSⁿ譜庫，使其成為用MS/MS進行多化合物篩選的性能優秀的離子阱質譜。

　　布魯克.道爾頓公司的副總裁Arnd Ingendoh 博士說：“amaZon將在廣泛的應用領域重新定義離子阱性能。無與倫比的靈敏度、采集速度和質量分辨率，加上易于使用、智能自動化操作以及一系列軟件模塊，可更快地提供高價值的信息。amaZon在許多小分子等應用領域提供了新的性能和確信度。而在蛋白質組學領域，amaZon成為一個非常強大的ESI-MS/MS系統，具有超高靈敏度、全自動的ETD/PTR，代表了一種和MALDI-TOF/TOF技術互補的全方位的蛋白質組學分析技術。”

　　革命性的新型amaZon質譜將在2009年5月31日、第57屆ASMS會議中的Bruker用戶會上亮相。

　　Bruker Redefines Ion Trap Mass Spectrometry with the Introduction of amaZon?, the Next-Generation Ultra-Sensitive Ion Trap

　　Source: BUSINESS WIRE

　　Monday, May 11, 2009

　　The Novel amaZon is the First Ion Trap with Dual Ion Funnel Technology for a 10x Sensitivity Boost, Second-Generation ETD for Proteomics, Mass Resolution up to 20,000, and Highest Acquisition Speed

　　BILLERICA, Mass.--(BUSINESS WIRE)--May. 11, 2009-- Bruker Daltonics today launches the next-generation ultra-sensitive, ion trap amaZon? series. Delivering 10x improved MS/MS sensitivity over current platforms, novel technology for accelerated data acquisition, significantly enhanced mass resolution, and second-generation fast and sensitive ETD/PTR capabilities, this unique, highest performance ion trap delivers unrivalled data quality and extreme flexibility for a broad variety of applications.

　　The amaZon builds upon Bruker’s long experience in ion trap technologies, and is designed to enhance capabilities in research areas such as bottom-up proteomics, high-throughput and ultra-sensitive PTM analysis, metabolomics, compound screening and identification, and detailed chemical and structural analysis. The amaZon series is offered in two versions: amaZon ETD for proteomics, and amaZon X for small molecule analysis and applied markets.

　　The new amaZon incorporates key inventions for unprecedented ion trap capabilities:

　　?First commercial introduction of a double ion funnel transfer in ion traps, delivering a new benchmark for sensitivity

　　?Unrivalled scan speed of 52,000 u/sec at better than unit resolution for fastest UPLC applications and maximum duty cycle in data-dependent MS/MS and MSn

　　?Ultimate ion trap mass resolving power up to 20,000 in full scan mode across the 50-3000 m/z range: in combination with Bruker’s patented peak recognition technology SNAP IITM, the amaZon provides isotopic peak resolution even for highly charged ion species in ETD/PTR fragment spectra for the confident determination of their monoisotopic masses.

　　?The latest ETD/PTR technology offering the most efficient, extremely robust and sensitive peptide and protein fragmentation, as well as high-throughput PTM analysis. Building upon the very successful ETD technology commercialized first by Bruker, the combination of bottom-up CID or ETD and top-down ETD/PTR provides new methodologies for detailed protein characterization.

　　?Zero Delay Alternating? acquisition for high-speed ion polarity switching without sensitivity and time compromise. Up to 20 Hz MS data acquisition in both polarities is ideal in combination with fast UHPLC separations. Supported by spectral MSn libraries, the amaZon is the ultimate ion trap mass spectrometer for MS/MS based multi-compound screening.

　　Bruker Daltonics Vice President Dr. Arnd Ingendoh commented: “The performance of the amaZon will completely redefine the use of ion traps across an increasing range of application areas. With new, unparalleled levels of sensitivity, acquisition speed and mass resolution combined with ease-of-use, smart automation and an array of software modules for faster high-value information generation, the amaZon offers a new level of performance and confidence in many small molecule and applied market segments. In proteomics, the amaZon is a very powerful ESI-MS/MS system with ultra-sensitive, fully automated ETD/PTR, and represents a perfect complement to MALDI-TOF/TOF technology for the broadest array of proteomic analysis capabilities.”

　　The revolutionary new amaZon will be highlighted at Bruker's Annual Users Meeting on Sunday, May 31st, 2009 before the 57th ASMS Conference on Mass Spectrometry in Philadelphia, PA. For more information on this event, please visit www.bdal.com/asms2009. For further product information please visit www.bdal.com/amaZon.

　　ABOUT BRUKER DALTONICS: For more information about Bruker Daltonics and Bruker Corporation (NASDAQ: BRKR), please visit www.bdal.com or www.bruker.com.