In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for light microscopical and ultrastructural autometallographic (AMG) studies. Specific methods were developed to demonstrate endogenous zinc pools in synaptic and secretory vesicles; exogeneous mercury, silver bound as sulfide or selenide crystals, in lysosomes; and tissue-b......閱讀全文
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization
OverviewThis protocol is a detail description of the laboratory procedure in performing 2D gel electrophoresis for illustrating the protein profile of
Overview This protocol is a detail description of the procedure in performing 2D gel electrophoresis for illustrating the protein p
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
I. EQUIPMENT:DNA sequencing unit (35 x 45 cm) & 2000V power supplyClampsLg. plastic trays (4), about 43 x 50 x 8 cm, and one lidTwo rocking p
Phytohemaglutinin (PHA)Available as kidney bean lectin. It is typically used as a stock solution of 10-20 g/ml in balanced salt solution. For tissue c
I. Safety ProceduresA. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are require
Sodium citrate (MW 294.10)0.09 MDissolve 2.65 grams of sodium citrate to a final concentration of 100 ml with water.Sodium citrate/formaldehyde (for s
下表列出的大部分(礦物)成分來自自然資源,它們的化學組成可能有差異,并且,許多原料是各向異性的。所有或其中之一的影響導致所表明的折射率值有一個范圍;非透明粒子的表面粗糙度常用假定折射率的虛部范圍在0.01i~0.1i進行計算。這樣,在下表中,帶星號(*)的這些材料由于文獻