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Hydrodynamic Analysis and EM Applied to Large Multisubunit ComplexesThe text box above showed the application of the Siegel–Monte analysis to SMC protein from B. subtilis, which had only one type subunit and was found to be a dimer. Similar hydrodynamic analysis can be used to analyze multisubunit protein complexes. There are many examples in the literature; I will show here an elegant application to DASH/Dam1.T......閱讀全文
Fig. 6HS-AFM observations of the non-specific transient binding of Cas9–RNA. a Sequential HS-AFM images of Cas9–RNA molecules transiently bound to
The innate immune response responds in a general manner to factors present in invading pathogens. Bacterial factors such as lipopolysaccharides (LPS,
Abstract Choriallantoic membrane (CAM) limb grafting functions as a method to isolate the inductive eve
Interleukin-1 (IL-1) is a pro-inflammatory cytokine that signals primarily through the type 1 IL-1 receptor (IL-1R1). The activities of IL-1 include i
IL-7 is a key cytokine in the immune system, essential for normal development of B cells and T cells. Mice with the IL-7 receptor deleted lack B and T
The inflammatory response associated with asthma is characterized by the recruitment of eosinophils from the bronchial microcirculation in response to
實驗概要 Extension of overlapping gene segments by PCR is a simple, versatile technique for site-directed mutagenesis and g
淋巴細胞活化基因3（Lymphocyte-activation gene 3 ,LAG-3, CD223）是一個在活化T細胞上發現的跨膜蛋白，其蛋白質胞外段有4個免疫球蛋白樣結構域 (D1–D4) ，和CD4分子具有較高的同源性【1】。LAG-3主要功能為作為受體傳遞抑制性信號【2】，負調控CD
Table 1: Hydrolysis of 125I-labeled Ub-PESTc by the purified YUH1.Specific activity againstDUBsCbz-LRGG-AMC125I-labeled Ub-PESTcYUH13.2 x 10-105.
實驗概要The PureLink? Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
The differentiation of muscle cells is transcriptionally regulated, in part by the myocyte enhancer factor-2, MEF2. During myogenesis MEF2 binds to My
Mitochondria Structure and Function - Theoretical DetailsIn an attempt to be concise and understandable, introductory level courses and textbooks freq
In an attempt to be concise and understandable, introductory level courses and textbooks frequently present concepts that are technically correc
The IL-2 receptor is a key component of immune signaling and is required for the activation, proliferation, and survival of T cells. This receptor is
The cytoplasm of nearly all eukaryotic cells contain mitochondria, although there is at least one exception, the protist Chaos (Pelomyxa) carolin
Eukaryotic cells take up constituents of the extra-cellular environment and regulate the cell-surface level of membrane proteins via a recycling syste
Lipid analysisEXTRACTION OF LIPIDS FROM LIPOPHORIN Since lipids are hydrophobic, they are better soluble in organic solvents than in water. Becau
The calcium/calmodulin-dependent kinases (CaMKs) are involved in a large number of cellular responses induced by hormones, neurotransmitters and other
One of responses to increased blood pressure is cardiac hypertrophy through increased size of ventricular myocardial cells leading to increased thickn
ResultsRNA-induced structural stabilization in Cas9We first observed apo-Cas9 and pre-assembled Cas9–RNA on a mica surface treated with 3-aminopro
Significant progress has been made towards delineation of the intrinsic molecular processes that regulate B lymphocyte immune function. Recent observa
1、Required Materials and Equipment(1) Protein A or G agarose gel column (10 ml or 5 ml of packed beads; see guidelines below for choice of protein A o
By: Christopher P. Toseland1 2 , Martin R. Webb1Affiliation(s): (1) MRC National Institute for Medical Research, London, UK(2
Primary referencestop American Journal of Clinical Pathology (AJCP), August 1975 to February 2006American Journal of Surgical Pathology
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
Liver is the major site for carbohydrate metabolism (glycolysis and glycogen synthesis) and triglyceride synthesis (lipogenesis). While insulin was lo
I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice animal by prescribed and ap
Cyclin-dependent kinase 5 (cdk5), a multi-functional kinase, and its neuron-specific activator p35 are required for neurite outgrowth and cortical lam
實驗概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
The hepatocyte growth factor receptor, also called c-Met, is activated by HGF and stimulates proliferation of hepatocytes and other cell types. Mutate