AMethodforAssayingDeubiquitinatingEnzymes1
AbstractA general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate. Since the tyrosine residue in the PESTc portion of the fusion protein was almost exclusively radioiodinated under a mild labeling condition, such as using IODO-BEADS, the enzymes could be assayed directly by simple measureme......閱讀全文
A Method for Assaying Deubiquitinating Enzymes-1
Abstract A general method for the assay of deubiquitinating enzymes was described in detail using?125I-labeled ubiquitin-fused αNH-MHISPPEPESE
A Method for Assaying Deubiquitinating Enzymes-2
Table 1: Hydrolysis of?125I-labeled Ub-PESTc by the purified YUH1.Specific activity againstDUBsCbz-LRGG-AMC125I-labeled Ub-PESTcYUH13.2 x 10-105.1 x 1
yeast:Assaying mating
Setup You have yeast strains that are deficient in mating (eg Ste12 knockouts) and would like to test whether transforming them with a plasmid that
creative-enzymes工業用酶
化學加工作為具有高反應速率的可持續催化劑，酶已引起了化學催化劑以外的更多關注。如今，由于清潔和高效的催化性能，越來越多的化學過程被酶所取代。例如，在手性合成中，酶顯示出極高的立體特異性，這是化學催化劑所沒有的，這簡化了整個合成過程。環境與廢物管理酶是天然催化劑，可選擇性分解較大分子的混合物。在酶技術
Method: Lymphocyte Transformation
Method: Lymphocyte Transformation May 30, 1990 Rosalie Veile Principle: Lymphocytes are transformed to establish cell lines. Mononuclear
內切酶列表：Enzymes Generating Blunt Ends
Asymmetric sequences are indicated by *. Single letter code: R = G or A;? Y = C or T;? W = A or T;? M = A or C;? K = G or T;? S = C or G; H = A, C
內切酶列表：Enzymes Generating Blunt Ends
Asymmetric sequences are indicated by *.Single letter code:R = G or A;?Y = C or T;?W = A or T;?M = A or C;?K = G or T;?S = C or G;H = A, C or T;V = A,
Easy YAC Preparation Method
YAC TRANSFORMATION OF C. ELEGANS USING TOTAL YEAST GENOMIC DNA [This method is described in The Worm Breeder's Gazette (1997) A. Davies and J. Shaw,
A Method for Structure-3
CSP-Dependent Transformation AssayTo determine if synthetic peptides activated quorum sensing for induction of genetic competence, we used the?comC?de
A Method for Structure-1
A Method for Structure–Activity Analysis of Quorum-Sensing Signaling Peptides from Naturally Transformable StreptococciMany species of streptococci se