DetectionbyTUNELlabeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809), modified by Josiah N. Wilcox,José C. Rodriguez, and Héctor de Léon - Emory University - April 1996Protocol for Paraffin Sections:Dewax paraffin sections:Incubate slides, 55°C, 30 min.Xylenes, 2 times, 2 min. each100% EtOH, 2 times, 2 min. each95% EtOH, 2 times, 2 min. each80% EtOH, 2 min.75% EtOH, 2 min.50% EtOH, 2......閱讀全文
Detection by TUNEL labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling by Boehringer Mannheim (Catalog No. 1684809), modified by Josiah N. Wilcox, José C. Rodrig
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling
Protocol for Frozen Sections: Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT. 1x PBS rinse, 2 times. 1x PBS, 30
TUNEL labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling by Boehringer Mannheim (Catalog No. 1684809), modified by Josiah N. Wilcox and José C. Rod
Guide to Cell Proliferation and Apoptosis Methods
Chapter 1: Cell Death - Apoptosis and Necrosis 1.1 Introduction 2 1.1.1 Terminology of cell death 2 1.1.2 Differences between necros
Labeling Tubulin and Quantifying Labeling Stoichiometry
Labeling Tubulin and Quantifying Labeling Stoichiometry This is a general procedure for coupling moieties with reactive succinimidyl esters to tubul
Labeling Tubulin and Quantifying Labeling Stoichiometry-2
II. Labeling ProtocolThe procedure described below can be scaled down if desired. It is essential to perform all steps involving caged dyes under a sa
Biosynthetic labeling
How long should cells be labeled?? The ideal length of time to label cells depends on the protein of interest and the label that you are using. If y
Coenzyme A Detection
實驗概要The experiment provided ?an ?easy, convenient assay to measure the CoA level in a variety of ?biological samples. In the assay, free CoA is specif
CMFDA Labeling of Platelet
OUTLINE CMFDA (5-chloromethylfluorescein diacetate) is a lipophilic tracer that has an enormous advantage over ordinary tracers (e.g. FITC) because
Immunofluorescence Labeling of Cells
實驗概要 Antibodies are an ?important tool for demonstrating both the presence and the subcellular ?localization of an antigen. Cell staining is a very