PREPARATIONOFSEQUENCINGGELS
MATERIALS:2-glass plates1 sharks -tooth comb and spacersWhatman 3 mm paper30 or 40% acrylamide-bis (19:1)10X TBEurea10% ammonium persulfateTEMED60 cc. syringeRain-exPreparation of glass plates:Wash and rinse well the sequencing plates. Silanize the smaller notched plate as follows. Apply a small amount of Rain-ex to the inside surface of the small plate with a Kimwipe. Let air dry to a thin film. Rinse well with dist......閱讀全文
PREPARATION-OF-SEQUENCING-GELS
MATERIALS:2-glass plates1 sharks -tooth comb and spacersWhatman 3 mm paper30 or 40% acrylamide-bis (19:1)10X TBEurea10% ammonium persulfateTEMED60 cc.
NO-WEDGE-Sequencing-Gels
I have been using an alternative to wedge gels that saves acrylamide, cuts gel drying time to 20 min and gives as good or better band squashing at the
DNA-Sequencing-Gels
DNA Sequencing GelsBuffers and gel solutionsLong Ranger: we started using this in early 1995. Great stuff; the best thing is that the gels are not sti
Preparation-of-Polyacrylamide-Gels
1. Prepare 20X TBE as: 216 g Tris Base 110 g Boric Acid 80 mL 500 mM EDTA, pH 8.0 700 mL ddH2O Mix. Bring volume to 1 L. Autoclave. 2. Prepare Acry
Sample-preparation-(analytical-gels)
Sample preparation and solubilization are crucial factors for the overall performance of the 2-D PAGE technique. Protein complexes and aggregates shou
Preparation-of-Agarose-Gels-for-DNA-separations
Weigh out the desired amount of agarose and place in an Erlenmeyer flask with a measured amount of electrophoresis buffer, e.g. for an 0.8% gel, add
Edman-Sequencing-of-Proteins-from-2D-Gels
The Western blotting/sequencing technique using polyvinylidene difluoride (PVDF) membrane is one of the most popular technique for Edman sequenci
Preparation-of-denaturing-6%polyacrylamide-gels-for-microsatellite-analysis
Preparation of denaturing 6% polyacrylamide gels for microsatellite analysis(also for SSAP, high-resolution IRAP/ISSR and other analyses)Saadiah Jamli
DNA測序
DNA測序(主要內容如下)·?????????Sequencing Gel Preparation·?????????Preparation of Templates?·?????????DNA Sequencing by the Dideoxy Method·?????????DNA Sequen
DNA電泳
DNA電泳(主要內容如下)??Preparation of Agarose Gel and Electrophoresis??Extraction of DNA From Agarose Gel??Extraction of DNA from Acrylamide Gels??DNA Marker?
NuPAGE-Gels
NuPAGE GelsA gel electrophoresis system used for SDS-PAGE protein analysis. The gels are made up of Bis-Tris-HCl (pH 6.4) polyacrylamide and are inten
DNA-mobility-in-gels
1. Migration of marker dyes in native polyacrylamide non-denaturing gels Gel?% Bromophenol?blue?(BP) Xylene?cyanole?(XC) ??3.5 ?100 460 ??5.0
蛋白質電泳
蛋白質電泳(主要內容如下)One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel?Gel StainingRecipesOne-Dimensional SDS-PAGE·??????
BAC-EndSequencing
BAC End-Sequencing (Diana Bocskai) For every 4 mls of culture, dissolve the BAC DNA pellet in 40 μl of water. for example: Usually each BAC is g
ELECTROPHORESIS-OF-DNA-IN-AGAROSE-GELS
ELECTROPHORESIS OF DNA IN AGAROSE GELSA). AGAROSE CONCENTRATIONS:???????Use 0.8% agarose (w/v) for high molecular weight DNA fragments, and 1 - 1.2% f
ELECTROPHORESIS-OF-DNA-IN-POLYACRYLAMIDE-GELS
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall:???? ????????165 x 130 mmMedium: ????????165 x 200 mmLarge:??? ????????165 x 260 mm5% Anal
DNA抽提
DNA抽提(主要內容如下)·???Working with DNA·???DNA Extraction from Bacteria and Other Organisms·???DNA Extraction from Cell and Tissue·???Mitochondria DNA Isola
Agarose-Gels-for-Single-Stranded-DNA
1. Prepare 50X TAE as: 242 g Tris Base 57.1 mL Glacial Acetic Acid 100 mL 500 mM EDTA, pH 8.0 600 mL ddH2O Mix. Bring volume to 1 L. Autoclave. 2.
Native-Acrylamide-Gels-(35-ml)
Native Acrylamide Gels (35 ml)3.5%5%6%8%30/0.8% acrylamide4.1 ml5.8 ml7 ml9.3 ml10X TBE3.5 ml3.5 ml3.5 ml3.5 mlH2027.4 ml25.7 ml24.5 ml22.2 mlDegas 1-
DNA-Purification-from-Agarose-Gels
1. Separate DNA fragments in an agarose gel cast with 0.5 mg/mL Ethidium bromide. Locate bands with a hand-held long-wave UV lamp.2. Slice the gel wit
Preparation-of-tubulin
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have
SMEAR-PREPARATION
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria
Platelet-Preparation
OUTLINEIn order to avoid platelet activation all manipulations must be performed as quickly and as acurate as possible.Work on ice if possible!?This p
Template-Preparation
Template PreparationThe quality of sequencing results is directlyrelated to the quality of the template.?ABI recommends a minialkaline-lysis/PEG preci
Liposome-Preparation
Liposome PreparationOBJECTIVE:Method for incorporating proteins into liposomes for liposome swelling assay or as an alternative antigen presentation m
CAM-preparation
8 eggs per day, day 7- day 13?cut CAM, wash in precooled PBS,in 10 ml WASH 1 (PBS, 5 mM EDTA, COMPLETE) on icecut in pieces in petri dish on icecentri
酵母準備
Yeast DNA PreparationYeast Genomic Preparation? (Gottschling Lab)Rapid method for yeast genomic DNA isolation??Yeast DNA Preparation (rapid glass bead
EGel?-CloneWell-Agarose-Gels
實驗概要Instructions are ?provided below for using the E-Gel?CloneWell pre-cast agarose gels with ?the E-Gel? iBase? Power System. For detailed instructio
Analysis-of-Proteins-using-Small-Format-2D-Gel-Electrophoresis
Preparation of protein samplesIntracellular virus proteinsThe following method has been developed principally for the analysis of intracellular protei
Preparation-of-human-platelets
Preparation of human platelets????? 1.?Human blood was taken from drug-free volunteers on the day of the experiment using acidic citrate dextrose