Sample preparation and solubilization are crucial factors for the overall performance of the 2-D PAGE technique. Protein complexes and aggregates should be completely disrupted in order to avoid appearance of new spots due to a partial protein solubilization. The SWISS-2DPAGE samples were prepared as followed:Human samplesCEC (Colon epithelial cell): Within a maximum of 30 min, a large right or left colon tissue samp......閱讀全文
As a primary component of circulatory system, serum is the major reservoir of thousands of proteins secreted or “leaked” from a broad spectrum of
一、儀器設備721、723可見分光光度計、752紫外可見分光光度計、UV-2401PC紫外可見分光光度計、UV-3150PC紫外可見近紅外分光光度計、RF-5301PC熒光分光光度計、比色液及標準溶液、濾紙等。 二、儀器結構三、原 理 光譜儀器是一種簡單易用的分光光度法測定通用儀器,不同型號的機器
實驗概要The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is c
Use of Non-aqueous Fractionation and Metabolomics to Study Chloroplast Function in ArabidopsisChloroplasts are the chemical factories of plant cells b
蛋白質電泳(主要內容如下)One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel Gel StainingRecipesOne-Dimensional SDS-PAGE·&
Tutorial III:樣品制備技術及反相高效液相色譜法固定相的選擇Tutorial III:Sample Preparation Techniques and Reverse Phase HPLC Stationary Phase Selection Tutorial III:樣品制備技術及反
Suitable for use on single-cell suspensions from peripheral blood, lymphoid tissue or cultured cell-lines.Sample Preparation and FixationHarvest cells
在生物藥肽圖分析、蛋白質修飾分析、蛋白質組學研究等諸多工作中,蛋白質的變性與酶解是樣品處理的必經之路。RapiGestTM SF正是在這個實驗步驟中,用以輔助蛋白酶解的變性試劑[1,2,3]。RapiGest SF有以下優點:■ 綜合水相、有機相溶液條件下,最高效的輔助酶解變性劑。■ 作用
生物通報道 Illumina公司近日宣布了一系列產品和技術上的創新,從樣品制備到系統改進再到數據分析,這有望帶來基因組研究上的新突破。隨著平臺的不斷改進,測序速度、準確性、產量和流程都有望改善。 樣品制備新品 簡化和加速樣品制備的過程對改善測序流程和降低周轉時間至關重要,這也是測
分析測試百科網訊 2019年9月1日,第四屆全國樣品制備學術報告會在青島銀沙灘溫德姆至尊酒店繼續召開。(相關報道:第4屆全國樣品制備學術會在青島召開 關注新機遇新挑戰,大會報告:簡化制樣、提高靈敏度 看第四屆全國樣品制備會大咖報告)在精彩的大會報告之后,各個專家、廠商紛紛帶來樣品制備方面的新材料
I. EQUIPMENT:DNA sequencing unit (35 x 45 cm) & 2000V power supplyClampsLg. plastic trays (4), about 43 x 50 x 8 cm, and one lidTwo rocking p
NuPAGE GelsA gel electrophoresis system used for SDS-PAGE protein analysis. The gels are made up of Bis-Tris-HCl (pH 6.4) polyacrylamide and are inten
第五屆金屬組學國際研討會會議日程 Scientific Programme Wednesday, 9th Sept 2015 9:00 -21:00 Registration Wednesday, 9th Sept 2015, Ginkgo Hall
2017年10月11日,第十七屆北京分析測試學術報告會暨展覽會期間,由實驗與分析平臺-弗戈工業媒體組織的2017樣品前處理前沿技術及行業應用論壇在北京·國家會議中心舉行。 多位來自科研院所及企業的嘉賓進行了精彩的報告。 國家環境分析測試中心首席專家、POPs研究室主任董亮博士的報告為“環境樣
IPAC 2012 Call for paper and Introduction The International Process Analysis & Control Congress, China 2012 (IPAC 2012) is the first event s
N-glycosylation modification of proteins has reported to improve the health of living organisms through antibacterial activity [68], antioxidant a
Why are proteins, detected by mass spectrometry, not validated by site-specific antibodies?The modified motif could be detected by mass spectrometry (
RJ provides efficient energetic fuels for the fast development of larvae and the egg-laying queen through the metabolism of sugars, lipids, and pr
NGS 是一種識別和確認未知致病菌的前景廣闊的技術,然而其在生物防御和公共健康應用等方面的時效性,卻往往因為缺乏快速、有效、可靠的自動DNA樣品制備方法而受到限制。為了突破這種限制,Kim 等設計了一種基于流體分布元件的數字微流體(DMF) 平臺,使得多子系統模塊能夠進入自動NGS庫樣品
據美國科學家分析,在未來一段時間后,土壤肥力評估將以遙感為主,輔以少量參比土樣分析。 在該階段實現以前(約5 ~ 10年),由于精細管理(Sitespecific management,SSM)和變量施肥(Variable ratefertilizer application,VRA)的要求,將有
一.實驗步驟1. 組織塊稱重2. 利用液氮、研缽粉碎組織塊3. 加入RIPA緩沖液(每克組織3 ml RIPA),PMSF(每克組織30μl,10 mg/ml PMSF),利用Polytron進一步勻漿(15,000轉/分*1分鐘)維持4℃4. 加入PMSF(每克組織30μl,10 mg/ml PM
實驗概要a protocol for Leaf GUS Assay This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samplesAs
實驗概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemical
Introduction Multi-cellular populations are fundamentally driven by the collective properties of individual cells. However, our unde
Biohit Liquid Handling is Now Part of Sartorius 百得液體處理部門現在屬于賽多利斯 We are pleased to report that Sartorius, a leading international process
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF
Introduction to Immunocytochemistry (House Ear Institute)A brief overview of common available methods. BrDU Immunocytochemistry using
實驗概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemical
分析測試百科網訊 2016年7月2日,中國化學會第30屆學術年會召開之際,分會之一質譜分析分會如期舉行。來自質譜分析界的專家、學者近200人參加了本次會議。整個會場座無虛席清華大學化學系教授、中國化學會質譜分析專業委員會的秘書長 林金明
· Double Peroxidase (HRP) Immunohistochemical Labeling of Trypsin-Sensitive Antigens (KPL)·