2DPolyacrylamideGelElectrophoresis
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study.SolutionsTIP: Use electrophoresis grade reagents to prepare the following solutions:A: 50 ml IEF Lysis BufferAdd 21 g urea to 35 ml HPLC-grade H2O to a 50 ml Falcon tube (final concentratio......閱讀全文
2-D Polyacrylamide Gel Electrophoresis
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailo
Polyacrylamide Gel Electrophoresis of Oligonucleotides
1. Pour and polymerize a 20% polyacrylamide gel, no Urea. 2. Remove clamps. Rinse with water. Remove comb. Rinse top of gel well. 3. Insert comb te
蛋白質電泳
蛋白質電泳（主要內容如下）One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel?Gel StainingRecipesOne-Dimensional SDS-PAGE·??????
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELS
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall:???? ????????165 x 130 mmMedium: ????????165 x 200 mmLarge:??? ????????165 x 260 mm5% Anal
聚丙烯酰胺凝膠電泳(polyacrylamide gel electrophoresis,PAGE)
配制 Tris- 甘氨酸 SDS-PAGE 聚丙烯酰胺凝膠電泳分離膠所用溶液 ? 溶液成分 不同體積（ ml ）凝膠液中各成分所需體積（ ml ） 5 10 15 20 25 30 4
DNA電泳
DNA電泳（主要內容如下）??Preparation of Agarose Gel and Electrophoresis??Extraction of DNA From Agarose Gel??Extraction of DNA from Acrylamide Gels??DNA Marker?
Agarose gel electrophoresis
General Procedure Cast a gel Place it in gel box in running buffer Load samples Run the gel Image the gel Casting Gels 0.7% ag
Gel Electrophoresis of DNA
What is Electrophoresis? Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab
Agarose Gel Electrophoresis
實驗概要 Separating nucleic acid fragments by agarose gel electrophoresis. 實驗原理 ? Agarose ?gel electrophoresis remains the most widely used technique
RNA gel electrophoresis
實驗概要RNA gel electrophoresis主要試劑DEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37oC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide sol