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MethodUsing sterile pipette tips, transfer a 1 mm colony into 50 uL of 60 U/ml Zymolyase3 uL of 1 U/mL Zymolyase stock solution47 uL of waterIncubate cell suspension at 37 C for 30 minutes.Incubate cell suspension at 95 C for 10 minutes.Use 5 uL of the cell suspension for a 50 uL PCR reactionZymolase Stock Solution10,000 U Zymolyase (Sigma)1 mL KHPO4 buffer, pH 7.5 (adjusted with KOH)0.2 mL 5M NaCl5 mL 100% glycerolA......閱讀全文
· Standard PCR Protocol (Molecular Biology Techniques Manual)The followings are described in
OverviewThis is a quick and easy yeast colony PCR protocol that does not require zymolyase step.Updated Protocol: Blackburn Lab: Quick and Easy Y
The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformat
DNA克隆（主要內容如下）· General Procedure· PCR Clonin
Colony PCR is useful in determining whether or not a specific colony on a plate has a sequence you desire. Primers for the specific sequence should be
OverviewThis is a method to assemble two BioBricks using the Clontech In-Fusion PCR Cloning Kit and maintains BioBrick standard formats. There are cur
PCR reactionProtocol for 50μl reaction - adjust amounts if necessary, for a 20μl reaction use the same volumes of primer and dNTP-mix, but adjust the
Bacterial Colony PCRObjective:This protocol allows rapid detection of transformation success when primers are available to allow determination of
MaterialsPCR SuperMix High FidelityVF2 primer (5''-TGCCACCTGACGTCTAAGAA-3'')VR primer (5''-ATTACCGCCTTTGAGTGAGC-3'')De
Description Polymerase Chain Reaction (PCR) is a method of amplifying a specific DNA target sequence. The cycle involves denaturing the template
2 kinds of newly launched products are available to SLAS Exhibitors to distribute on 2013 SLAS Asia Conference & Exhibition website. This
Cloning PCR products using TA vectorsby Paul N. Hengen, Ph.D. *Methods and reagents is a unique monthly column that highlights current discussion
6. Simultaneous digestion of the pUC vector with both enzymes in the presence of 3 units of Shrimp Alkaline Phosphatase (
DNA轉化Chemical Transformation· Transformation of Competent Cells (RbCl2 Method) (Goldberg Lab
Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18, 19961. An average-size yeast colony (0.5-2mm) or a cell pel
Transposon-mediated MutagenesisStep 1 - Amplify ORF from MG16551.0 ul genomic DNA (
Colony PCRDavid AmbergThis procedure will work for both yeast and E. coli:Take a small colony and suspend it in 5ul of H2O in a PCR tube. Heat fo
Large Scale Preps: (See Large scale plsasmid prep protocol for more details)Cultures: Inoculate a 5 mL LB/Amp (50 - 100 μg/mL) culture in early a
實驗方法原理 菌落PCR（Colony PCR）可不必提取基因組DNA，不必酶切鑒定，而是直接以菌體熱解后暴露的DNA為模板進行PCR擴增，省時少力。使用載體上的通用引物，進行重
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IQuantizatio
菌落PCR標簽： 菌落 PCR菌落PCR（Colony PCR）可不必提取基因組DNA，不必酶切鑒定，而是直接以菌體熱解后暴露的DNA為模板進行PCR擴增，省時少力。建議使用載體上的通用引物。通常利用此方法進行重組體的篩選或者DNA測序分析。最后的PCR產物大小是載體通用引物之間的插入片斷大小。
In the polymerase chain reaction (PCR), a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
菌落PCR（Colony PCR）可不必提取基因組DNA，不必酶切鑒定，而是直接以菌體熱解后暴露的DNA為模板進行PCR擴增，省時少力。建議使用載體上的通用引物。通常利用此方法進行重組體的篩選或者DNA測序分析。最后的PCR產物大小是載體通用引物之間的插入片斷大小。具體方法：1、PCR混合物的制備T
Zymolyase Solution:Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store aliquots
MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURERoutine Culturing of ES CellsISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTSMITOMYCIN C TREATMEN
Human zinc fingers as building blocks in the construction of artificial transcription factorsKwang-Hee Bae1, 4, Young Do Kwon1, 2, 4, Hyun-Chul Shin1,
五、核酸分子雜交的類型 隨著基因工程研究技術的迅猛發展，新的核酸分子雜交類型和方法在不斷涌現和完善。核酸分子雜交可按作用環境大致分為固相雜交和液相雜交兩種類型。固相雜交是將參加反應的一條核酸鏈先固定在固體支持物上，一條反應核酸游離在溶液中。固體支持物有硝酸纖維素濾膜、尼龍膜、乳膠顆粒、磁珠和微孔板
Introduction The most important aspect of our cloning vectors is that t
五、核酸分子雜交的類型 隨著基因工程研究技術的迅猛發展，新的核酸分子雜交類型和方法在不斷涌現和完善。核酸分子雜交可按作用環境大致分為固相雜交和液相雜交兩種類型。固相雜交是將參加反應的一條核酸鏈先固定在固體支持物上，一條反應核酸游離在溶液中。固體支持物有硝酸纖維素濾膜、尼龍膜、乳膠顆粒、磁珠和微孔板