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Grow 10ml YPD cultures o/n. Figure out cell density; inoculate 30 ml YPD and grow o/n so that cell density is approximately 2 x 108cells/ml the next morning.Spin down cells in 50 ml sterile conical tubes for 10 min. Centrifugation steps are performed at 4 degrees; all other steps are carried out at rm. temp. unless otherwise specified.Resuspend cells in 10 ml water and spin down cells.Resuspend cells in 3 ml of 0.9 M......閱讀全文
Yeast DNA PreparationYeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (r
實驗概要The PureLink? Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Correspon
Genomic DNA libraries Size of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bac
YAC TRANSFORMATION OF C. ELEGANS USING TOTAL YEAST GENOMIC DNA[This method is described in The Worm Breeder's Gazette (1997) A. Davies and J. Shaw
What is degenerate PCR? Degenerate PCR is in most respect identical to ordinary PCR, but with one major difference. Instead of using
CDNA文庫（主要內容如下）· Construction of cDNA Library· &nbs
Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. Instead of using specific PCR primers with a given sequen
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-fi
Purpose:To isolate intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yi
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
Key points to observe: a. Use a endA1- E. coli strain for plasmid propagation and isolation whenever possible. The instabilit
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
實驗概要DNAzol? Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and
· Standard PCR Protocol (Molecular Biology Techniques Manual)The followings are described in
實驗概要The PureLink? Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
實驗概要Quantitative PCR involves co-amplification of two templates: a constant amount of a preparation containing the desired target sequence and var
Quantitative PCRJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W. RussellUniversity of Texas Southwes
Genomic DNA Prepfrom 5 ml culture, resuspend in 50 μl TEDigestionsUse either AciI, AluI, HaeIII, HpaII, RsaI or TaqI for Leu transposon libraries37 de
Genomic DNA Prepfrom 5 ml culture, resuspend in 50 μl TEDigestionsUse either AciI, AluI, HaeIII, HpaII, RsaI or TaqI for Leu transposon libraries37 de
生命極其脆弱，我們每天在電子輻射、紫外線、霧霾等等各種外部環境及細胞代謝產物等內源因素影響下，我們生命的核心-DNA都會受到不同程度的損傷，其中DNA雙鏈斷裂（DSBs，Double strand breaks）是損傷中最為嚴重的一種，然而生命卻又極其強大，我們無時無刻不在受傷，也無時無刻不
【干貨】拯救你受傷的DNA-NHEJ與HR生命極其脆弱，我們每天在電子輻射、紫外線、霧霾等等各種外部環境及細胞代謝產物等內源因素影響下，我們生命的核心-DNA都會受到不同程度的損傷，其中DNA雙鏈斷裂（DSBs，Double strand breaks）是損傷中最為嚴重的一種，然而生命卻又極
文庫種類Dharmacon酵母資源包括多個酵母基因組文庫，包括ORF文庫、基因敲除（Knock Out）菌株、蛋白質相互作用文庫、突變菌株和各種篩選文庫等。除此之外，Dharmacon 針對Saccharomyces cerevisiae 研究領域提供了Zoonome siRNA 文庫。酵母
二、DNA標記與細胞譜系示蹤發育生物學的重點包括構成器官或生物體的細胞類型的多樣性以及這些細胞的發育譜系歷史。這些方面通常作為一個方向被單獨研究，但最近的四篇新論文報道了一種將單細胞RNA測序（scRNA-seq）技術與基于CRISPR的譜系示蹤技術相結合來同時解剖轉錄組細胞表型和譜系歷史的方法。近
自1995 年成立以來，Dharmacon 提供各種用于批量研究基因功能的工具，支持最多從全基因組范圍到信號通路、基因家族方面研究基因與疾病、表型、分子機理對應的關系。近年來隨著高端酶標儀、高通量顯微鏡、自動化流式細胞儀、高內涵等設備的普及和技術更新，Dharmacon 文庫在各個領域中
1. The following gel electrophoresis conditions are recommended:- use 1X TAE buffer instead of 1X TBE- use agarose gel in the concentration of 1.1%-1.
實驗概要RNA analysis on non-denaturing agarose gel electrophoresis實驗步驟1. The following gel electrophoresis conditions are recommended:- use 1X TAE buffer
· Protocols for Making Drosophila Arrays (Stanford U.)Detailed protocol for making arrays in