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The isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes have marked capacity for proliferation, self-renewal, and differentiation. These cells represents a minority of the tumor cell population and are identified by expression of the cell surface marker the positive CD133. These positive CD133 cells, termed the brain tumor stem cells (......閱讀全文
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
截至2019年12月23日，中國學者在Cell，Nature及Science在線發表了107篇文章（2019年的Cell ，Nature 及Science 已經全部更新），iNature團隊對于這些文章做了系統的總結： 按雜志來劃分：Cell 發表了31篇，Nature 發表了44篇，Scie
截止2020月7月27日，中國學者在Cell，Nature 及Science 發表了共計102項生命科學的研究成果，其中新冠肺炎領域占了近一半（共43篇）。iNature系統總結了這些研究成果： 按雜志來劃分：Cell 發表了30篇，Nature 發表了45篇，
Isolation of AE Cells1. Human placentae were obtained with the approval of the institutional review board, after uncomplicated elective caesa
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal stem cells in tissues and the la
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
可靠的CCCadvanced FN1無異源耗材支持人間充質干細胞擴增表達Reliable and Robust Animal-Component-Free hMSC-BM Expansion on Ready-to-Use Eppendorf CCCadvanced? FN1 Motifs Surf
多功能單細胞顯微操作系統FluidFM BOT在活細胞提取中的應用由于細胞異質性的存在，單細胞層面的分析就變得十分重要。目前對于單細胞分析的方法主要還是通過化學、生物學的方法進行裂解后，提取內容物進行分析，然而這種方法往往會對樣本造成一些損傷。直接提取活細胞具有諸多優點，但是操作苦難。如今一種全新使
由于細胞異質性的存在，單細胞層面的分析就變得十分重要。目前對于單細胞分析的方法主要還是通過化學、生物學的方法進行裂解后，提取內容物進行分析，然而這種方法往往會對樣本造成一些損傷。直接提取活細胞具有諸多優點，但是操作苦難。如今一種全新使用FluidFM科技的技術新報道有望提供一種活細胞提取新型的簡易方
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
實驗概要Neural stem cells (NSC) are valuable resources because of their ability to differentiate into neurons and glial cells with application
當前腫瘤治療的難點和研究重點有：腫瘤藥物耐受、腫瘤遷移和腫瘤復發，很多情況下腫瘤干細胞（Cancer Stem Cells, CSCs）是罪魁禍首【1,2】。腫瘤干細胞是一群分裂相對不活躍、成瘤能力強，并有高干細胞特性的腫瘤細胞，現已在造血系統的腫瘤、乳腺癌、前列腺癌、胰腺癌、結腸癌、皮膚癌和腦
Isolation and Transcription Profiling of Purified Uncultured Human Stromal Stem CellsIsolation and Transcription Profiling of Purified Uncultured Huma
在免疫學實驗中，比如WB，IHC，IF等，大家都知道選著好正確的一抗，往往實驗就成功了一大半，因此，往往很多研究者便會忽視對二抗，以及其它試劑盒品質的追求。二抗是一種相對而言比較穩定的產品，包裝大，因此很多實驗室都會公用一個二抗。但如果二抗沒有選擇好的話，影響的可能就是一整個實驗室或者課題組了。艾美
Genomic DNA libraries Size of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bac
實驗概要DNAzol? Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and
【51/52】2019年4月4日，清華大學柴繼杰課題組、中科院遺傳發育所周儉民課題組和清華大學王宏偉課題聯合同期背靠背發表兩篇重量級Science文章，完成了植物NLR蛋白復合物的組裝、結構和功能分析，揭示了NLR作用的關鍵分子機制，是植物免疫研究的里程碑事件。兩篇文章分別是： "Li
實驗概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
CD7Membrane glycoprotein and Fc receptor for IgM Homologous to TCR gamma, Ig kappaMembrane expression early during T ontogeny, before TCR rearran
實驗概要Directed differentiation of specific lineages has been a focal point in the field of human embryonic stem cell (hESC) research. Cell r
Noninvasive Human Nuclear Transfer with Embryonic Stem CellsSohyun L. McElroy1 and Renee A. Reijo PeraCenter for Human Embryonic Stem Cell R
EPC Isolation and Characterization1. EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human bloo
Stem cell di?erentiation assaysAdMSCs were harvested from both shake and spinner ?asks into 50 ml tubes. Once the microcarrier beads settled to the
實驗概要Rat neural stem cells (NSCs) serve as a well-established model for investigating human brain development, disease processes, and treat
日 期 時 間 會 議 內 容 11月10日 08:30-17:00 會議注冊 11月11日 08:30 集體照 08:30-12:00 開幕式和主題論壇 12:00-13:00 午餐
Cancer Cell：腫瘤干細胞為根治癌癥帶來了新的曙光。 癌癥是以細胞異常增殖及轉移為特點的一大類疾病。2000年全球新發癌癥病例約1000萬人，死亡620萬人；預計2020年癌癥新發病例將達到1500萬人，死亡1000萬人。《中國癌癥預防與控制規劃綱要2004-2010》指出：癌癥正在成為2
Figure 5: hMSC proliferation rate during long-term expansion in di?erent animal-component-free culture systemshMSC expansion on the FN1 motifs surface