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    • 分析測試百科網

    RetosBuffers&MediaBook

    發布時間：2019-04-22 23:21 原文鏈接： RetosBuffers&MediaBook

    08-15 Buffer

    Compound
    MW
    Final concentration

    200 ml

    Glycine75.07 
    0.1 M

    1.5 g

    1 M MgCl2
     10 mM

    2 ml

    KOH
    to pH 9

    H2O

    		to 200 ml

    A

    Acetic Acid 1 M

    Compound
    MW
    Final concentration

    50 ml

    Acetic Acid 98% (~17 M)
    1 M

    3 ml

    H2O

    to 50 ml

    Acrylamide/Bisacrylamide

    Compound
    MW
    Final concentration

    500 ml

    Acrylamide
    71.08
    20 %

    100 g

    Bisacrylamide
    154.17
    0.8 %

    4 g

    H2O

    to 500 ml


    Alkaline Lysis Buffer A

    Compound
    MW
    Final concentration

    500 ml

    Glucose
    180
    50 mM

    4.5 g

    Tris
    121
    25 mM

    1.5 g

    EDTA 0.25 M
    10 mM

    20 ml

    H2O

    to 450 ml

    Adjust pH 8.0 with HCl


    H2O, Autoclave, store at 4°C

    to 500 ml


    Alkaline Lysis Solution 1

    Compound
    MW
    Final concentration

    100 ml

    Lysozyme
    0.2 mg/ml

    0.02 g

    Alkaline Lysis Buffer A

    to 100 ml


    Alkaline Lysis Solution 2

    Compound
    MW
    Final concentration

    50 ml

    SDS 20 %
    1 %

    2.5 ml

    NaOH 10 M
    0.2 M

    1 ml

    H2O

    to 50 ml


    Alkaline Lysis Solution 3

    Compound
    MW
    Final concentration

    50 ml

    KAc
    98
    3 M

    14.7 g

    Acetic Acid 100 %
    11.5 %

    5.75 ml

    H2O

    to 50 ml


    Amido Black

    Compound
    MW
    Final concentration

    100 ml

    Amidoblack
    98
    0.1 %

    0.1 g

    Acetic Acid 100 %
    7.5 %

    7.5 ml

    Methanol
    20 % (v/v)

    20 ml

    H2O

    to 100 ml


    Aminoacid Concentration in Media

    Constituent
    Final mg/l
    Stock

    ml stock for 1 liter medium

    Adenine sulfate
    20
    2 mg/ml
    10
    Uracil
    20
    2 mg/ml
    10
    L-tryptophan
    20
     2 mg/ml
    10
    L-histidine-HCl
    20
    2 mg/ml
    10
    L-arginine-HCl
    20
    2 mg/ml
    10
    L-methionine
    20
    2 mg/ml
    10
    L-tyrosine
    30
    3 mg/ml
    10
    L-leucine
    30
    3 mg/ml
    10
    L-isoleucine
    30
    3 mg/ml
    10
    L-lysine-HCl
    30
    3 mg/ml
    10
    L-phenylalanine
    50
    5 mg/ml
    10
    L-glutamic acid
    100
    10 mg/ml
    10
    L-aspartic acid
    100
    10 mg/ml
    10
    L-valine
    150
    15 mg/ml
    10
    L-threonine
    200
    20 mg/ml
    10
    L-serine
    400
    40 mg/ml
    10

    3-Aminophtalhydrazide 250 mM

    Compound
    MW
    Final concentration

    10 ml

    3-Aminophtalhydrazide
    177
    250 mM

    442 mg

    H2O

    to 10 ml


    Ammoniummolybdate 4.2 %

    Compound
    MW
    Final concentration

    50 ml

    Ammoniummolybdate x 4H2O
    236
    4.2 %

    2.1 g

    H2O

    to 30 ml

    HCl fuming 37 % (~12 M)
    4 M

    16.7 ml

    H2O

    to 50 ml

    Ampicillin 20 mg/ml pH 8.0

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    • Compound
    MW
    Final concentration

    15 ml

    Ampicillin
    349.4
    20 mg/ml

    300 mg

    H2O

    to 10 ml

    Adjust pH 8.0 with NaOH


    H2O, Filter sterili

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