Day -1: Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.
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Day 1: Trypsinized the cells as for normal passaging until the colonies lift off. Try to keep the loosely connected clumps of cells together by gentle handling. Then directly plate the cells 1:3 into bacterial grade Petri dishes in LIF free ES cell medium.
Day 3: Aspirate the medium carefully. Avoid sucking up too many of the aggregates. Then add new medium.
Day 5: Aspirate as in Day 3 and replace the medium. At this stage the differentiation the aggregates are called simple embryoid bodies.
Day 7: Change the medium
Day 9: Change the medium
Day 11: Change the medium
Day 13: Change the medium Day 15: By this day a high percentage of the embryoid bodies will have developed fluid filled cavities. The structures are called cystic embryoid bodies
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研究人員發現了小鼠體內一種新的轉錄因子可以幫助調節間充質干細胞（mesenchymalstemcells，MSCs）分化為骨的過程。目前科學家們對于骨細胞分化的研究并不深入，而MSCs則是再生醫學領域......
經過幾十年的努力，科學家最終成功地從牛身上獲得胚胎干（ES）細胞，并在培養皿中使其保持原始狀態。獲得這些可變成從皮膚到肌肉、骨頭等各種組織的多功能細胞，將使調整和保存肉牛以及乳牛品種的遺傳性狀變得更加......
