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  • 發布時間:2020-07-21 10:35 原文鏈接: 45種培養基配方(細菌培養基與植物培養基)(四)

    28. Yeast Extract Peptone (酵母膏、蛋白胨瓊脂)
      Yeast extract (酵母膏) 1g Multi-peptone(多蛋白胨) 2g
      Beef extract (牛肉膏) 1g Glucose (葡萄糖) 10g
      Agar (瓊脂) 20g Distilled water (蒸餾水) 1L
      pH 7.0 


      29. Glucose Yeast Extract Starch Agar (葡萄糖、酵母、淀粉瓊脂)
      Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g
      Starch (淀粉) 10g NaCl 5g
      CaCO3 3g Agar (瓊脂) 20g
      Distilled water (蒸餾水) 1L pH 7.0 


      30. Wheat Bran Medium (麩皮培養基)
      Wheat bran (麩皮) 36g (NH4)2HPO4 10g
      K2HPO4 0.2g MgSO4·7H2O 0.1g
      Agar (瓊脂) 20g Distilled water (蒸餾水) 1L
      pH 7.0

    31. Yeast Sporulation Medium (酵母菌產生子囊孢子培養基)
      (1) Kleyn Medium
      KH2PO4 0.012% K2HPO4 0.02%
      Sodium acetate (醋酸鈉) 0.5% Glucose (葡萄糖) 0.062%
      NaCl 0.062% Peptone (蛋白胨) 0.25%
      Biotin (生物素) 2μg% Agar (瓊脂) 2%
      Trace element solution (混合鹽類) 1ml/100ml
      [Note]: Trace element solution (混合鹽類溶液): MgSO4.7H2O 0.4%, NaCl 0.4%,
      CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2%
      (2) Gorodkowa Medium
      Glucose (葡萄糖) 0.1% Peptone (蛋白胨) 1%
      NaCl 0.5% Agar (瓊脂) 2%
      (3) McClary Medium
      Glucose (葡萄糖) 0.1% KCl 0.18%
      Yeast juice (酵母汁) 0.25% Sodium actate (醋酸鈉) 0.82%
      Agar (瓊脂) 1.5% 


      32. BPY Medium (BPY 培養基)
      Beef extract (牛肉膏) 5g Prptone (蛋白胨) 10g
      NaCl 5g Yeast extract (酵母膏) 5g
      Glucose (葡萄糖) 5g Agar (瓊脂) 1-2%
      Distilled (蒸餾水) 1000ml pH 7.0
      適用范圍:大腸埃希氏菌(大腸桿菌)、惡臭假單胞菌 


      33. LB Medium (LB 培養基)
      Yeast extact (酵母膏) 5g Peptone (蛋白胨) 10g
      NaCl 10g Agar (瓊脂) 1-2%
      Distilled water (蒸餾水) 1000ml pH 7.0
      適用范圍:大腸埃希氏菌(大腸桿菌) 


      34. Penssay Broth (Penssay 肉湯)
      Beef extract (牛肉膏) 1.5g Yeast extract (酵母膏) 1.5g
      Tryptone (胰蛋白胨) 5g NaCl 3.5g
      Glucose (葡萄糖) 1g K2HPO4.3H2O 4.8g
      Distilled water (蒸餾水) 1000ml pH 7.2 


      35. Blond Agar (血瓊脂培養基)
      Peptone (蛋白胨) 10g Glucose (葡萄糖) 2g
      NaCl 5g NaH2PO4 2.5g
      Sodium citrate (檸檬酸鈉) 5g Brain infusion (腦浸汁) 800ml
      Pig heart infusion (豬心浸) 200ml Sleep Blood (羊血) 10%
      pH 7.2
      [Note]:Preparation of pig brain and pig heart infusion:
      (1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled
      water up to 1000ml;
      (2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to
      1000ml;
      (3) Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes;
      (4) Cool and filtrate through gauze .
      [注]豬心和豬腦浸汁法:(1)新鮮豬心除去心頭和脂肪,攪碎100克加蒸餾水至1000毫升;(2)新鮮豬腦除去血管,取200克加蒸餾水至1000毫升;(3)50℃保溫1小時,然后煮開15分鐘;(4)冷卻后用紗布過濾備用。

      36. Cellulose﹣decomposing Bacteria Synthetic Medium (纖維細菌合成培養基)
      NaCl 6g MgSO4.7H2O 0.1g
      KH2PO4 0.5g CaCl2 0.1g
      K2HPO4 2.0g Yeast extract (酵母膏) 1.0g
      (NH4)2SO4 2.0g Distilled water (蒸餾水) 1000ml
      Filter paper strip: one strip (濾紙條:一片) pH 7.0-7.4
      [Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl
      Cellulose 2% Agar 2%. (斜面培養基去濾紙條用2%瓊脂及2%羧甲基纖維素鈉 CMCNa )


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