AuxinImmunolocalizationinPlantTissues
The major naturally occurring auxin, indol-3 acetic acid (IAA), coordinates many growth and differentiation processes by modulating gene expression during plant development. The sites of IAA biosynthesis and its polar transport (PAT) routes determine auxin accumulation and distribution during growth. From many studies on the model plant Arabidopsis thaliana over the last years, it has become evident that th......閱讀全文
Auxin Immunolocalization in Plant Tissues
The major naturally occurring auxin, indol-3 acetic acid (IAA), coordinates many growth and differentiation processes by modulating gene expressio
Protein Immunolocalization in Maize Tissues
The ?analysis of gene expression at transcript and at protein level is of ?outstanding importance in plant developmental biology. Proteins can be ?loc
Rapid DNA Isolation from Phyllanthus Amarus and Other Plant Tissues
Procedure Preheat Extraction Buffer at 60°C. Weigh 100 mg of fresh leaf tissue and grind it to powder in Liquid Nitrogen in a chilled
Quantifying Kinetics of Net Ion Fluxes from Plant Tissues by Non-invasi...
Non-invasive microelectrode ion flux measuring (the MIFE system) allows concurrent quantification of net fluxes of several ions with high spatial
Extraction of RNA from Fibrous tissues
實驗概要 E.Z.N.A.? ?MicroElute? Total RNA Kit provides a rapid and easy method for the ?isolation of up to 50 ug of total RNA from small amount of cultu
Protein extraction from whole tissues for IEF
Modified from that of Jay Thelen - University of Missouri-Columbia Phenol extraction followed by methanolic ammonium acetate precipitation - an effe
FIXATION OF PLANT METAPHASE CHROMOSOMES
Reagents ?(a) Metaphase arresting agents: Choose one of the following (Note 2) and shake vigorously to aerate before putting in living plant mat
PCR from Plant Tissue
1.protocol (1)collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH (2)put in boiling H2Ofor 30
Plant Genome Annotation Methods
Annotation of plant genomic sequences can be separated into structural and functional annotation. Structural annotation is the foundation of all
PCR from Plant Tissue
PCR from Tissue?Reference:??Klimyuk et.al., 1993, Plant J. 3:493-494?Last updated: 1/27/00?By: Kay Schneitz?? ? ???collect piece of tissue (e.g., piec