WIKI資訊
A significant amount of metabolic energy can come from amino acid metabolism, particularly under conditions of starvation. The metabolism of amino acids occurs through common metabolic intermediates, many of them part of or linked to the Krebs cycle. The intermediates like pyruvate, oxaloacetate, fumarate, succinyl-CoA and alpha-ketobutyrate all can contribute to the net synthesis of glucose through gluconeogenesis. ......閱讀全文
Amino acids in the diet have one of two fates - either they are incorporated into proteins or they are broken down for energy and metabolic intermedia
(五)高氨血癥和氨中毒 正常生理情況下，血氨處于較低水平。尿素循環是維持血氨低濃度的關鍵。當肝功能嚴重損傷時，尿素循環發生障礙，血氨濃度升高，稱為高氨血癥。氨中毒機制尚不清楚。一般認為，氨進入腦組織，可與α酮戊二酸結合成谷氨酸，谷氨酸又與氨進一步結合生成谷氨酰胺，從而使α酮戊二酸和
An Introduction to BiocomputingVersion 2.01October 1996David Steffen, Ph.D.President, Biomedical Computing, Inc.6626 WestchesterHouston, Texas 77
Screening Kinase Phosphorylation Motifs Using Peptide LibrariesIsaac A. Manke and Michael B. YaffeCenter for Cancer Research, Massachusetts Institute
Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. Instead of using specific PCR primers with a given sequen
What is degenerate PCR? Degenerate PCR is in most respect identical to ordinary PCR, but with one major difference. Instead of using
Yeast Media:Note: Synthetic complete medium can be prepared by adding media supplements (see below).Medium using 6.7 g yeast nitrogen base without ami
Genomic DNA libraries Size of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bac
Reprinted from:Hansen, P.J and Betts, J.G. (1992) Culture of endometrial explants and peri-implantation conceptuses to monitor synthesis and secretion
Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and ProstaglandinsP. J. Hansen and J.
There has been a recent revival of interest in the use of AA composition for the identification of proteins from 2-D gels. This technique uses the idi
Excess amino acids in the body can be used as a source of energy, with their carbon skeleton converted to metabolic intermediates such as acetyl-CoA o
Synthesis and Probing of Membrane-bound Peptide ArraysRonald FrankDepartment of Chemical Biology, GBF (German Research Center for Biotechnology), 3812
LEVEL IIIMaterialsSuspension culture of fibroblast cells (1 liter)35 mM Tris-HCl, pH 7.4, 140 mM NaCl (TBS buffer)10 mM Tris-HCl, pH 7.5, 10 mM KCl, a
Organic substances.pKa and temperature dependence of pH for common buffers.ATP 0.1MBetaine 5MCresol red (Na) 50mMDTT 1M, 2.2M
Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh
PurposeThe protocol describes how to prepare selenomethionyl (Se-Met) protein using a regular E.coli strain. Selenium can be used for phase determinat
AbstractA general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEE
We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a
Lowry – Protein Determination(From Protein Protocols on CD-ROM Humana Press, 1998 - Section 1-2 The Lowry Method for Protein Quantitation Jakob H. Wat
Materials(Solutions are all available from the media room)200ml bottle of 2x SD200ml bottle of 4% agar -- make sure to sign it out40% glucoseCSM minus
Human zinc fingers as building blocks in the construction of artificial transcription factorsKwang-Hee Bae1, 4, Young Do Kwon1, 2, 4, Hyun-Chul Shin1,
2020年2月28日，據歐盟食品安全局（EFSA）消息，應歐盟委員會要求，歐盟動物飼料添加劑和產品（FEEDAP）研究小組就一種L-胱氨酸（L-Cystine）作為所有動物品種的飼料和飲用水的營養添加劑和感官添加劑的安全性與有效性發表科學意見。 據了解，這種L-胱氨酸是由菠蘿泛菌 （Panto
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
實驗概要Preparation of lysis buffers, protease and phosphatase inhibitors, lysate from cell culture, lysate from tissues, protein concentratio
Sodium orthovanadate preparationAll steps to be performed in a fume hood. a. Prepare a 100 mM so
Take the appropriate set of Fmoc-amino acid stock aliquots for cycle 1 from the freezer, bring to room temperature, and activate by adding DIC (4 μl p
Thyroid epithelial cells (also called follicular cells or principal cells) are cells of thyroid gland that are responsible for the production and
4 THz 光譜在水環境中生物分子研究的應用 水對于生物分子發揮其功能有著至關重要的作用，但長期以來，由于實驗儀器和研究方法的局限，水分子與生物大分子的相互作用難以觀察. 90年代后期，隨著THz-TDS技術開始應用于生物學研究領域，研究人員發現THz對生
MaterialsYPD platesYPD1 M sorbitol 182 g/l (Sigma S7547)2 M sorbitol 36.4 g/100 mlSCE (per liter)1 M sorbitol (182 g)100 mM citric acid trisodium salt