YeastMedia,SolutionsandStocks
Yeast Media:Note: Synthetic complete medium can be prepared by adding media supplements (see below).Medium using 6.7 g yeast nitrogen base without amino acids (Difco # 0919-15) can also be prepared using 1.7 g yeast nitrogen base without amino acids and ammonium sulfate (Difco #0335-15) and 5.0 g ammonium sulfate.AHC Medium (a selective medium for growing yeast strain AB1380, a host for YACs)6.7 g yeast nitrogen......閱讀全文
Yeast Media, Solutions and Stocks
Yeast Media:Note: Synthetic complete medium can be prepared by adding media supplements (see below).Medium using 6.7 g yeast nitrogen base without ami
Bacterial Media Solutions and Stocks
3 agar?(200 ml)Add 6 grams agar to 200 ml deionized water. Autoclave to sterilize.1.6 agar?(200 ml)Add 3.2 grams agar to 200 ml deionized water.Autocl
Yeast Media
YEPD (non-selection)-1% yeast extract-2% peptone-1.5% agar (if needed for plates)After autoclaving add glucose to 2% by adding 100 ml of 20% solution
Cell Culture Media and Solutions
Cell Culture Media and Solutions Dec. 18, 1990 R. Veile Antimycotic/antibiotic media: To 1 liter of sterile RPMI 1640 with 2mM L-gl
Cell Culture Media and Solutions
Antimycotic/antibiotic media: To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add: 165.0 ml fetal bovine serum, heat inactivated 12.0 m
酵母培養基和相關試劑
Yeast Hartwell's Complete (HC) Media?(Gottschling Lab)??Yeast Complete Media???Yeast Media?(PMCI)??Yeast Media, Solutions and Stocks?(Donis Keller
TISSUE CULTURE STOCK SOLUTIONS AND MEDIA
MS MEDIUM FOR ARABIDOPSIS To?990?ml?H2O?add: Sucrose?...........?10.0??g MOPS?..............??0.5??g Agar?..............??8.0??g Adjust?pH?to?
MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE
Media UsedTo prepare 100 ml mediumDMEM80 mlFCS15 mlNon-essential amino acids (100x)1 mlPen/strep (5,000 1U/ml, 5000 ug/ml)1 mlL-Glutamine 200 mM1 mlNu
酵母培養
Streaking Yeast Stocks?(Donis Keller Lab)Very nice protoocol for yeast workLong-Term Storage of Yeast Stocks?(Donis Keller Lab)Yeast storage and reviv
細菌培養基
Preparation of LB Plate?(Dr. Chastain)prepare LB plate with or without antibioticsBacterial Culture Media Recipes?(WUGSC)?M9 Plate Supplement?(Gottsch