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INTRODUCTIONFlow cytometry is frequently used to assess nucleic acid content in individual cells. Based on DNA content alone, however, cells in the quiescent G0 phase cannot be discriminated from cellsin the proliferative G1 phase, as DNA content remains constant until S-phase entry. In contrast, by measuring RNA content in addition to DNA content, cells can be assigned to G0 and ce......閱讀全文
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 μg/mL of 7-AAD. Incub