CombinedFlowCytometricMeasurementofTwoCellSurfaceAntigens2
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 μg/mL of 7-AAD. Incubatefor 20 min at 20°C-25°C, protected from light. ii. Prepare a cell sample that is stained only with 7-AAD (without any cell surface staining). This sample is necessary to determine the amount of fluorescence overlap of 7-AA......閱讀全文
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens
INTRODUCTION Flow cytometry is frequently used to assess nucleic acid content?in individual cells. Based on DNA content alone, however, cells?in the
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens-2
DNA and RNA Staining 6. Stain cells with 7-AAD:? i. Resuspend the cells from Step?5 in 0.5 mL of NASS containing?10 μg/mL of 7-AAD. Incu
Detection of Intracellular Antigens by Flow Cytometry
實驗概要Fix and Perm ?reagents are designed for use with all commercially available flow ?cytometers. Alignment and compensation should be performed accor
Flow Cytometric Analysis of Cell Cycle
Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells
Flow Cytometric Analysis Of Bcl Family members
DescriptionCell Fixation, staining and flow cytometric analysis?ProcedureCells (106) were washed twice in FACS buffer (phosphate buffered saline PBS p
Staining Procedure for Flow Cytometric Detection of Human Cyclins
Staining Procedure for Flow Cytometric Detection of Human CyclinsThis is a standard protocol used at Pharmingen for Quality Control testing of the ant
碳水化合物分析
Carbohydrate Assay?(Hancock Laboratory) (Accessible only by IE) This protocol is used to determine the relative amounts of LPS CHO present in a giv
流式細胞儀技術專輯
Flow Cytometry Analysis?(Springer Lab, Harvard University)? Flow cytometry employs instrumentation that scans single cells flowing past excitation
流式細胞儀技術專輯
?最方便的實驗干貨查詢工具微信掃碼進入「丁香實驗」小程序編輯：?嗚咽分享到：??????Flow Cytometry Analysis?(Springer Lab, Harvard University)Flow cytometry employs instrumentation that scan
免疫熒光
Immunofluorescence Technique?(Spector Lab)protocol for immunofluorescence on cells??Immunofluorescence Protocol?(Walter Steffen)Methanol fixationForma