ELISA:Direct
實驗概要The following protocol provides a method of direct ELISA by Peprotech.主要試劑Tween-20 (Sigma Cat. # P-7949) BSA (Sigma Cat. # A-7030) ABTS Liquid Substrate Solution (Sigma Cat. # A3219) Dulbecco’s PBS [10x] (Gibco BRL Cat. #14200-075) All solutions should be at ambient temperature prior to use. PBS: Dilute 10xPBS to 1xPBS, pH 7.20 in sterile water. Wash Buffer: 0.05% Tween-20 in PBS Block Buffer: ......閱讀全文
ELISA: Direct
實驗概要The following protocol provides a method of direct ELISA by Peprotech.主要試劑Tween-20 (Sigma Cat. # P-7949) BSA (Sigma Cat. # A-7030)? ABTS Liquid Su
Direct/Indirect Staining Protocol
Use this Protocol for Directly or Indirectly staining cells for Flow Cytometric Analysis 1) Dilute cells to 5x10^6 cells/mL 2) Aliquot 100uL of ce
Direct PCR from Whole Yeast Cells: Zymolyase Method
Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18, 19961. An average-size yeast colony (0.5-2mm) or a cell pel
DIRECT AND SHORT-TERM PROCEDURE FOR HARVESTING BONE MARROW CHROMOSOMES
I. PurposeTo identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleuke
全血直接PCR試劑盒Blood Direct PCR Kit
全血直接PCR試劑盒Blood Direct PCR Kit全血擴增引物設計注意事項1）引物3’端zui后一個堿基選擇C或G；2）引物3’端zui后8個堿基應避免出現連續錯配；3）引物3’端盡量避免出現發夾結構；4）引物Tm值控制在60℃-72℃之間 (推薦使用軟件Primer Premier 5進
ELISA
Gangliosides ELISA protocol?(Contributed by?pingsunjim)This protocol can be used for detection of gangliosides.Specific antibodies to gangliosides are
Competitive ELISA
DAY 11. Coat Nunc immuno-module plates overnight, in usual manner.2. Set up competition assay between antibody and competing substance :-Prepare a 1:2
ELISA直接法和間接法優缺點
直接法（direct ELISA）將抗原直接固定在固相載體上，加入酶標記的一級抗體，即可測定抗原總量，此一級抗體的特異性非常重要。優勢：操作手續簡短，因無須使用二抗可避免交互反應。缺點：試驗中的一抗都得用酶標記，但不是每種抗體都適合做標記，費用相對提高。間接法（indirect ELISA）此測定方
乙酰化蛋白快速檢測和定量
Rapid detection and quantitation of total cellular acetylated proteins using our research products
ELISA
ELISA是一種經典的測定液體樣本中蛋白含量的方法。該方法優點在于可以準確定量蛋白含量，且測定樣本的種類可以多種多樣。本期小編帶你一起了解ELISA不同樣品的制備方法。細胞培養上清液移取細胞培養基至離心管中，4℃ 下以 1500 rpm 的速度離心 10 分鐘。立即分裝上清液，并將樣品儲存在 -80