InPlantaTransformationofArabidopsis
實驗概要 A breakthrough in Arabidopsis research was the invention ofthe vacuum-infiltration procedure, a simple and reliable methodof obtaining transformants at high efficiency while avoidingthe use of tissue culture. The plant transformation proceduresdescribed here involve floral dip, vacuum infiltration, andspraying. They yield transformants at frequencies ranging upto several pe......閱讀全文
Transformation Protocol for Arabidopsis
Transformation Protocol for Arabidopsis – Abbreviated Germinate seed in pots ↓ 4 weeks Streak bacteria onto YM/MinA ↓ 2-3 days 28°C Spray/dip ba
Arabidopsis gDNA isolation
This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons. We only us
In Planta Transformation of Arabidopsis
實驗概要? ? ? ? A breakthrough in Arabidopsis research was the invention ofthe vacuum-infiltration procedure, a simple and reliable methodof obtaining
Arabidopsis RNA extraction protocol
1-2 g fresh material, freezer-dried, ground with 0.2g sand (if necessary), and then homogenized with 10ml RNA extraction buffer (see below).
Arabidopsis RNA extraction protocol
1-2 g fresh material, freezer-dried, ground with 0.2g sand (if necessary), and then homogenized with 10ml RNA extraction buffer (see below). Spi
Cell Suspension Culture of Arabidopsis
實驗概要 Cell Suspension Culture of Arabidopsis? 主要試劑 10% (v/v) Household Bleach Callus Induction Medium? ? ? ?Gamborg's B5 Basal Medium? ? ? ?0.5
Simplified Arabidopsis Transformation Protocol
實驗概要Our present protocol (Clough and Bent, 1998; modified from Bechtold et al. 1993) is extremely simple. We have found that the MS salts, hormone
Simplified Arabidopsis Transformation Protocol
(Brief version for those who are familiar with the method)Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign.Our present proto
Production of Antibody Fragments in Arabidopsis Seeds
Plants offer a number of attractive benefits over conventional mammalian or bacterial cell culture systems for the production of valuable pharmac
ChIP using plant samples – Arabidopsis
實驗概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T