AbC?AntiMouseBeadKit
實驗概要The AbC? Anti-Mouse Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies (Figure 1). The kit contains two types of specially modified polystyrene microspheres, the AbC? capture beads, that bind all isotypes of mouse immunoglobulin, and the negative beads that have......閱讀全文
AbC?－AntiMouse－Bead－Kit
實驗概要The ?AbC? Anti-Mouse Bead Kit provides a consistent, accurate, and ?simple-to-use technique for the setting of flow cytometry compensation ?when u
AbC?－AntiMouse－Bead－Kit
實驗概要The ?AbC? Anti-Mouse Bead Kit provides a consistent, accurate, and ?simple-to-use technique for the setting of flow cytometry compensation ?when u
AbC?－AntiRat/Hamster－Bead－Kit
實驗概要The AbC? ?anti-Rat/Hamster Bead Kit provides a consistent, accurate, and ?simple-to-use technique for the setting of flow cytometry compensation ?
AbC?－AntiRat/Hamster－Bead－Kit
實驗概要The AbC? ?anti-Rat/Hamster Bead Kit provides a consistent, accurate, and ?simple-to-use technique for the setting of flow cytometry compensation ?
LIVE/DEAD?－Fixable－Dead－Cell－Stain－Kits
實驗概要The ?LIVE/DEAD? Fixable Dead Cell Stain Kits use a novel method to evaluate ?the viability of mammalian cells by flow cytometry. These assays are
LIVE/DEAD?－Fixable－Dead－Cell－Stain－Kits
實驗概要The LIVE/DEAD? ?Fixable Dead Cell Stain Kits use a novel method to evaluate the ?viability of mammalian cells by flow cytometry. These assays are
臨床輸血ABC
? 輸血作為一種成熟的醫療手段，在臨床上發揮了極其有效甚至關鍵的作用。許多臨床實踐證明，對于某些疾病，輸血已經成為治病救人的必要選擇。但許多患者對血液和臨床輸血存在著種種疑慮和誤解，所以有必要做一些澄清和講解。??? A.親屬間輸血并不安全。有人認為病人輸用親屬的血液最安全，這種說法是不對的。從某種
MicroRNA－Expression－Profiling－by－Bead－Array－3
Table?2?Modulation of microRNA expression by IFNα—4 and 24?h after stimulation ? Control Interferon-alpha treated
MicroRNA－Expression－Profiling－by－Bead－Array－1
MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2aMicroRNAs are positive and negative r
MicroRNA－Expression－Profiling－by－Bead－Array－4
Bead-array-based microRNA detection technology, including the bio-statistic analysis, is currently not well established or widely used and we have app
MicroRNA－Expression－Profiling－by－Bead－Array－2
Materials and MethodsCell Culture, Interferon Treatment, and RNA PrecipitationMelanoma cells (ME-15) were cultured in RPMI 1640 with L-Glutamine suppl
Optimized－Fibrin－Gel－Bead－Assay－for－the－Study－of－Angiogenesis
PREPARING CELLSBring up HUVEC and fibroblasts in M199/10% FBS/Pen-Strep (1:100) 1-2 days before beading.Switch medium to EGM-2 (Clonetics) the day bef
ProcartaPlex－免疫檢測常見問題及解決方案
eBioscience公司Luminex液相蛋白芯片檢測試劑——ProcartaPlex可以為您的生物學研究提供各種多因子檢測方案，包括細胞因子、生長因子、趨化因子和其他蛋白。在選擇試劑盒及操作時會遇到很多問題，現將常見問題及答復匯總如下，供您參考： 如果不小心將整個試劑盒保存在-20°C. 這個試
Miniprep/Qiagen－kit
MaterialsFor purifying plasmid DNA from?Escherichia coli?cells, the Qiagen Spin Miniprep Kit produces quite reliable results.Do not autoclave solution
ABCELISA材料和方法
(以篩選抗空腸彎曲菌共同抗原的單克隆抗體為例) 1 材料 (1) 空腸彎曲菌共同抗原提取物，自制。 (2) 生物素化羊抗鼠IgG(AMG)，衛生部上海生物制品所。 (3) ABC試劑，衛生部上海生物制品所。如ABC試劑需自己配制，可按說明要求，將親和素與酶標生物素按一定比例混合，置3
ABC－轉運器的結構特點
ABC轉運器（ABC transporter）最早發現于細菌，是細菌質膜上的一種運輸ATP酶（transport ATPase），屬于一個龐大而多樣的蛋白家族，每個成員都含有兩個高度保守的ATP結合區（ATP binding cassette），故名ABC轉運器，他們通過結合ATP發生二聚化，ATP
Dynabeads?－CoImmunoprecipitation－Kit
實驗概要The ?Dynabeads? and buffers provided in this kit will enable you to a) ?covalently immobilize antibodies of your choice onto the surface of ?Dynab
Dynabeads?－CoImmunoprecipitation－Kit
實驗概要The ?Dynabeads? and buffers provided in this kit will enable you to a) ?covalently immobilize antibodies of your choice onto the surface of ?Dynab
Dynabeads?－Antibody－Coupling－Kit
實驗概要Non-specific ?binding of an antibody to proteins other than the antigen can sometimes ?occur. This is usually more common with polyclonal antibodi
Human－DC－Enrichment－Kit
實驗概要Enrich ?untouched Dendritic Cells (DCs) by depleting T cells, B cells, ?monocytes/ macrophages, NK cells, erythrocytes and most granulocytes ?from
Dynabeads?－Antibody－Coupling－Kit
實驗概要The ?Dynabeads and buffers provided in this kit will enable you to ?covalently immobilize antibodies (or other protein ligands such as ?lections,
Dynabeads?－Streptavidin－Trial－Kit
實驗概要Intended UseDynabeads? ?Streptavidin are ideal for numerous applications, including ?purification of proteins, nucleic acids purification, protein
免疫組化ABC法步驟
1、4%多聚甲醛常規灌注固定，取材并置20%蔗糖溶液（4℃）中過夜。蠟塊制作。切片，貼片。0.01MKPBS清洗5min×3后；2、加入配好的0.3%的過氧化氫甲醇溶液（甲醇80ml+0.01MKPBS 100ml+30%過氧化氫）30min，以消除內源性過氧化物酶的影響，0.01MPBS清洗5mi
頂空進樣器知識ABC
頂空進樣器是氣相色譜法中一種方便快捷的樣品前處理方法，其原理是將待測樣品置入一密閉的容器中，通過加熱升溫使揮發性組分從樣品基體中揮發出來，在氣液（或氣固）兩相中達到平衡，直接抽取頂部氣體進行色譜分析，從而檢驗樣品中揮發性組分的成分和含量。使用頂空進樣技術可以免除冗長繁瑣的樣品前處理過程，避免有機溶劑
Brdu免疫組織化學染色分析－BrdU－incorporation－assay
Enzyme Immunostaining for BrdU:Wash frozen sections with PBS 2x.Put them in 0.1% Pepsin in 0.1N HCL (in PBS) at 37?C for 50min.Then in 0.3% hydrogen p
iPrep?－GeneCatcher?－gDNA－Blood－Kit
實驗概要The iPrep? ?GeneCatcher? gDNA Blood Kit allows rapid and automated extraction of ?genomic DNA (gDNA) from human blood including archived or poorly
Dynabeads?－Streptavidin－Trial－Kit（二）
3. General Immobilization Protocol??????????? Wash the Dynabeads? before use.??????????????????a)????? Add the biotinylated molecule to the washed Dyn
Dynabeads?－Streptavidin－Trial－Kit（一）
實驗概要Dynabeads? ?Streptavidin are ideal for numerous applications, including purification ?of proteins, nucleic acids purification, protein interaction
CellTrace?－CFSE－Cell－Proliferation－Kit
實驗概要The CellTrace? ?CFSE Cell Proliferation Kit provides a versatile and well-retained ?cell-tracing reagent in a convenient and easy-to-use form. The
免疫組化ABC法操作流程
1、4%多聚甲醛常規灌注固定，取材并置20%蔗糖溶液（4℃）過夜，蠟塊制作，切片，貼片。0.01M PBS清洗5min×3次；2、脫蠟、水化：用二甲苯兩次10min，用梯度乙醇由低濃度到高濃度進行水化；3、加入0.3%的過氧化氫甲醇溶液（甲醇80ml＋0.01M PBS 100ml＋30%過氧化氫）