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MATERIALSMedia:Heat inactivated FBS (56°C x 30 min)PBS + 2% heat-inactivated FBSIMDM + 20% heat-inactivated FBSViral transduction: Iscove's + 20% heat inactivated FBS,plus 100 ng/mL each of SCF, IL-6, and Flt-3L, plus 10 ng/mL IL-11.CFU assays: aMEM +30% FBS, 1% deionized BSA, 1.2% methylcellulose, 0.1mM ?ME.plus 3 U/mL Epo, 50 ng/mL Tpo, 100 ng/mL SCF, 100 ng/mL IL-6, ? ng/mL IL-3.Antibodies for Lineag......閱讀全文
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
Protocol 3: Fetal Liver-Derived HSC Differentiation on OP9-DL1 CellsDay 0: Initiation of Fetal Liver Co-culture52. Isolate liver tissue from eight to
TROUBLESHOOTINGProblem: The OP9 cells are more than 80%-90% confluent.Solution: It is important when creating working stocks of OP9 cel
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
Figure 5: hMSC proliferation rate during long-term expansion in di?erent animal-component-free culture systemshMSC expansion on the FN1 motifs surface
I. PurposeTo identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleuke
Harvesting traumatized muscle derived mesenchymal progenitor cells (MPCs)1. The muscle tissue was dissected without contaminating by granulation,
41. Schmidt O, Theopold U, Strand M: Innate immunity and its evasion and suppression by hymenopteran endoparasitoids. BioEssays 2001, 23(4):344–35
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral VectorsEls Verhoeyen and Fran?ois-Lo?c CossetAdapted from Gene Tra
可靠的CCCadvanced FN1無異源耗材支持人間充質干細胞擴增表達Reliable and Robust Animal-Component-Free hMSC-BM Expansion on Ready-to-Use Eppendorf CCCadvanced? FN1 Motifs Surf
Materials and MethodsShort-term cell growth evaluationLonza? Poietics? human mesenchymal stem cells (hMSC-BM, PT-2501, Lonza) derived from normal ad
4. 培養方法的選擇 本文列出迄今為止最常用的三種BMDC培養方法，我們從下表中可以更清楚地看出這三種方法的異同，相信您能根據您的需要作出正確的判斷。
Bone marrow contains three types of stem cells:1. Hematopoietic stem cells give rise to the three classes of blood cells that are found in the
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho
在免疫學實驗中，比如WB，IHC，IF等，大家都知道選著好正確的一抗，往往實驗就成功了一大半，因此，往往很多研究者便會忽視對二抗，以及其它試劑盒品質的追求。二抗是一種相對而言比較穩定的產品，包裝大，因此很多實驗室都會公用一個二抗。但如果二抗沒有選擇好的話，影響的可能就是一整個實驗室或者課題組了。艾美
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
Human primary cells are cells taken from living human beings and cultured. These cells retain the differentiation of the original cells take
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal stem cells in tissues and the la
Materials and Methods1. Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tis
造血干細胞（Hematopoietic stem cells, HSCs）是一群具有自我更新能力和分化成各類成熟血細胞潛能的成體干細胞。自上世紀六十年代，McCulloch和Till共同發現和定義造血干細胞（詳見BioArt報道：被遺忘的干細胞研究先驅丨致敬Ernest McCulloch和Ja
注：點擊文章名稱查看詳情。文章名稱樣本儀器型號備注Assessment of the Genotoxic Potential of Azidothymidine in the Comet,Micro
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
Intestinal intraepithelial lymphocytes Intestinal intraepithelial lymphocytes (IELs) are mostly T cells, which are continuously
The inflammatory response associated with asthma is characterized by the recruitment of eosinophils from the bronchial microcirculation in response to
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA
Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et al. 1999), we have systematically analyzed t
The siRNA user guide (revised May 6, 2004) Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysa
DescriptionProcedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. ProcedurePreparing Culture Surface- Collag