IsolationandCultureofHumanLiverStemCells
Materials and Methods1. Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tissue (5–20g) was used to isolate hepatocytes by collagenase digestion.2. Liver tissues were isolated and perfused with 350 ml of a warm (37°C) calcium-free buffer.3. Liver tissues were digested in Digest Medium at 37°C. This resulted in blanching, softening......閱讀全文
Isolation－and－Culture－of－Human－Liver－Stem－Cells
Materials and Methods1.?Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tissue (
Isolation－and－Culture－of－Multipotent－Stem－Cells－from－Human－Bone－Morrow
Bone marrow contains three types of stem cells:1.?Hematopoietic stem cells give rise to the three classes of blood cells that are found in the circu
Culture－of－Human－Embryonic－Stem－Cells－(hESC)
All cell lines are initially grown according to the supplier's protocols but we are adapting them to one simple protocol outlined below:6-well pla
Isolation－and－Culture－of－Human－Brain－Tumor－Stem－Cell
The isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes have marked capacit
Isolation－and－Culture－of－human－vascular－smooth－muscle－cells
1.?Human vascular smooth muscle cells?(SMCs) were isolated from human saphenous vein.2.?Small moistened pieces of tissue (6 mm2) were placed intima
Isolation－and－culture－of－human－pulmonary－artery－smooth－muscle－cells－...
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs)1.?The human pulmonary arteries were opened to expose the endothelial su
Isolation－of－human－multipotent－mesenchymal－stem－cells－from－second
Isolation of human multipotent mesenchymal stem cells from second‐trimester amniotic fluid?Culture of MSC from amniotic fluid1.?Twenty amniotic flui
Ex－Vivo－Human－Primary－Mesenchymal－Stem－Cells－(MSCs)－Culture
Human bone marrow contains mesenchymal progenitors (mesenchymal stem cells, MSCs).? MSCs produce adventitial cells in the human bone marrow microenvir
Human－B－cell－isolation－and－culture
B cell isolation1.?Donor blood was obtained with informed consent under guidelines issued by the Medical Ethics Committee. 2.?Mononuclear cell fractio
Human－B－cell－isolation－and－culture
實驗概要This protocol provides a general protocol for human B cell isolation and culture.實驗步驟B cell isolation1.?Donor blood was obtained with informed con
Isolation－and－culture－of－pancreatic－stellate－cells
Pancreatic stellate cells (PaSCs or PSCs) are myofibroblast-like cells found in the areas of the pancreas that have exocrine function.? PaSCs are
Isolation－and－culture－of－porcine－muller－cells
1.?Porcine eyes were harvested from pentobarbital-anesthetized animals, each weighing 30 to 40 pounds, and transported to the laboratory in ice-co
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous SystemScott R. Hutton?and?Larysa H. Pevny1UNC Neuroscience Center
Isolation－of－human－atrial－or－ventricular－cells
1.?Human tissue was derived from atrial or ventricular biopsy specimens belonging to patients undergoing heart surgery.2.?Isolated myocardial tissue w
Isolation－of－human－endometrial－epithelial－cells
Tissue collection1.?Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2.?All women reported
Isolation－of－human－prostatic－epithelial－cells
1.?A small piece of tissue from each specimen was removed and minced. 2.?The tissue was digested with collagenase overnight. 3.?To remove the collagen
Isolation－of－Human－Pulmonary－Epithelial－Cells
?Isolation of Human Pulmonary Epithelial Cells????1.?Pulmonary epithelial cells from human lung tissue were isolated.2.?Lungs were perfused with 10
Culturing－Human－Neural－Stem－Cells
實驗概要Neural ?stem cells (NSC) are valuable resources because of their ability to ?differentiate into neurons and glial cells with applications in ?neur
Culture－of－human－venous－smooth－muscle－cells
Culture of human venous smooth muscle cells???1.?Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared
Isolation－of－human－corneal－endothelial－cells－(HCECs)
Isolation of HCECs1.?The corneoscleral tissues were rinsed three times with primary cell culture system containing 50 mg/mL gentamicin and 1.25 mg
Isolation－of－human－colonic－epithelial－cells－(EC)
1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were confirmed by clini
Isolation－of－human－prostatic－smooth－muscle－cells
Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of the prostate. 2.All
Isolation－of－human－uterine－smooth－muscle－cells
1.?Approval to utilize human tissue was given by Hospital's Committee on Research Involving Human Subjects. 2.?Human myometrial and leiomyoma tiss
Isolation－and－culture－of－rat－coronary－microvascular－endothelial－cells
CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1.?Hearts mounted on a Langendorff apparatus were perfused at 3
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells2
Slide Coating13. Just before use, dilute the poly-D-lysine stock solution?1:50 in H2O. Add 0.2 mL of diluted poly-D-lysine to each well?of an eight-we
Isolation－of－human－primary－gastric－mucosa－epithelial－cells
1.?A piece of gastric mucosa (～3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.?The patients were proven to be no H.
Isolation－of－human－primary－ovarian－surface－epithelial－cells
1.?Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institutional Review (References)
Xenofree－Culture－of－Neural－Stem－Cells
實驗概要Neural ?stem cells (NSCs) derived from human embryonic stem cells (hESCs) have ?the potential to help provide understanding for human neurogenesis
Noninvasive－Human－Nuclear－Transfer－with－Embryonic－Stem－Cells
Noninvasive Human Nuclear Transfer with Embryonic Stem CellsSohyun L. McElroy1?and?Renee A. Reijo PeraCenter for Human Embryonic Stem Cell Research an
Human－Embryonic－Stem－(ES)－Cell－Protocols——Thawing－Human－ES－cells
Remove Human ES cells from liquid nitrogen storage tank. Fill out a freeze/thaw form.Thaw cryovial by gently swirling in waterbath until only a small