體外熒光法檢測核內體早期動力學3
REAGENT SETUPCritical step All solutions and buffers must be free of detergent and therefore should be prepared in plasticware, or in glassware thoroughly cleaned with ethanol. All reagents that can be stored in aliquots at ?20 °C can be used for at least 6 months after preparation.PC12 culture mediumThe medium consists of DMEM supplemented with 10% (vol/vol) horse serum, 5% (vol/vol) fetal calf serum, 4 mM glut......閱讀全文
體外熒光法檢測核內體早期動力學-3
REAGENT SETUPCritical step?All solutions and buffers must be free of detergent and therefore should be prepared in plasticware, or in glassware thorou
體外熒光法檢測核內體早期動力學
A fluorescence-based?in vitro?assay for investigating early endosome dynamicsSina V Barysch1,2, Reinhard Jahn1?& Silvio O Rizzoli2ABSTRACTEarly endoso
體外熒光法檢測核內體早期動力學-5
Leave tubes on ice and repeat Steps 13–15 for the next 6–12 plates of cells.When all cells are collected, centrifuge all tubes at 250g?for 5 min at 4
體外熒光法檢測核內體早期動力學-4
Top?of pageProcedureOverviewSteps 1 - 8 Preparation of rat brain cytosolSteps 9 - 10 Culture of PC12 cellsSteps 11 - 30 Preparation of postnuclear sup
體外熒光法檢測核內體早期動力學-2
Full size image (70?KB)In vitro?incubation of a reaction mix that contains labeled endosomes, cytosol and an ATP-regenerating system at physiological
體外熒光法檢測核內體早期動力學-6
Critical step?Be careful to thaw PNS and cytosol slowly on ice to avoid unwanted protein degradation or breaking of organelles. This might require up
流式應用精選——微核檢測（體內&體外）
?注：點擊文章名稱查看詳情。文章名稱樣本儀器型號備注Assessment?of?the?Genotoxic?Potential?of?Azidothymidine?in?the?Comet,Micronucleus,?and?Pig-a?AssayBone?Marrow?and?Peripheral
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