DisruptionbyFusionPCR
Disruption by Fusion PCRDavid Amberg and Ellen Beasley1) In separate PCR reactions, amplify the 5' and 3' ends of the gene of interest with primers about 200 bases apart. Primer 2 should begin with 24 nts complementary to the m13 forward primer(GTC GTG ACT GGG AAA ACC CTG GCG) and primer 3 should begin with 24 nts complementary to the m13 reverse primer (TCC TGT GTG AAA TTG TTA TCC GCT). PCR amplif......閱讀全文
Disruption by Fusion PCR
?Disruption by Fusion?PCRDavid Amberg and Ellen Beasley1) In separate PCR reactions, amplify the 5' and 3' ends of the gene of interest with p
Cell Disruption and Subcellular Fractionation
Nitrogen Bomb1) Harvest cells and wash 2 times with PBS.2) Resuspend final pellet in relaxation buffer. (20 ml/1X109 cells)3) Pressurize with N2 for 2
Fusion and Cloning
Author:?Nanci DonackiSource:?Contributed by Nanci DonackiAbstract:?Procedure for establishing hybridoma in one stepReagents(StemCell Technologies, Inc
Fusion and Cloning
ReagentsMedium A - Pre-fusion Medium and Hybridoma Expansion MediumMedium B - Fusion Medium Medium C - Hybridoma Recovery MediumMedium D - Hybridoma S
Protocol for Cell Fusion
Healthy Sp2/0 cells should be rapidly growing by this time. Sp 2/0 cells should be started about two weeks before the cell fusion. Every two days, the
In-fusion biobrick assembly
OverviewThis is a method to assemble two BioBricks using the Clontech In-Fusion PCR Cloning Kit and maintains BioBrick standard formats. There are cur
酵母遺傳學技術
Genome-wide Gene Expression Analysis?(Richard Young Research Group,Whitehead Institute for Biomedical Research)A genoe-wide gene expression analysis u
PRCC基因編碼的功能和結構描述
這個基因編碼一種蛋白質，可能在前mrna剪接中起作用。染色體易位（X；1）（p11；q21）導致該基因與TFE3（基因id 7030）融合，與乳頭狀腎細胞癌有關PRCC-TFE3融合蛋白在癌組織中表達，可能與基因反式激活改變有關這種融合蛋白也與細胞周期的破壞有關。This gene encodes
Bacterial Expression of GST-fusion Proteins
1.? Grow cells in 5ml (or more) of LB-Amp overnight for a starter culture. ? 2.? Grow larger culture (100x volume of starter culture) using the ove
Thrombin Cleavage of GST-Fusion protein
INTRODUCTIONIn many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix.?The