Isolationandgrowthofmouseprimarymyoblasts
Isolation of limb muscle from neonatal mice1. Neonatal mice by decapitation or CO2 inhalation.2. Rinse the limbs with 70% ethanol and remove them with sterile scissors. Dissect the muscle away from the skin and bone with sterile forceps.Dissection is easier if done under a stereo dissecting microscope. Store the muscle tissue in a culture dish on ice in a drop of sterile PBS as successive limbs ar......閱讀全文
Isolation and growth of mouse primary myoblasts
Isolation of limb muscle from neonatal mice1.?Neonatal mice by decapitation or CO2 inhalation.2.?Rinse the limbs with 70% ethanol and remove them wi
ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
You will need:13.5 day pregnant mouse (we use MTK NEO inbred white mice)2 sets sterile instrumentsone containing a pair of curved forceps and a pair o
Isolation of mouse embryos
1. Sacrifice impregnated mouse. 2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forceps, use another to tear the mes
RAT/MOUSE GROWTH HO...
實驗概要This ?Rat/Mouse Growth Hormone ELISA kit is used for the non-radioactive ?quantification of Growth Hormone in rat or mouse serum, plasma, tissue ?
Primary cardiac fibroblast and cardiomyocyte isolation
1.?Ventricles were removed under sterile conditions. 2.?Ventricles were placed in cold sterile primary cell culture medium, minced into approximat
Primary brain cell isolation and culture
1.?Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containing 20% (v/
Isolation and growth of pulmonary artery adventitial fibroblasts
1.?Adventitia from the main pulmonary artery was harvested neonatal calves. 2.?Tissue was collected, carefully dissected free of blood vessels and
Isolation of human primary airway smooth muscle
1.?Primary cultures of human primary airway smooth muscle (ASM) cells were prepared from explants of airway smooth muscle. 2.?The human trachea tiss
Primary type II pneumonocyte Isolation and culture
Pulmonary alveolar type II cells carry out highly specialized functions that include the synthesis, secretion, and reutilization of surfactant, a
Isolation of human primary gastric mucosa epithelial cells
1.?A piece of gastric mucosa (～3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.?The patients were proven to be no H.