PouringPlates
1. For rich media, weigh out appropriate ingredients and place into a flask. Add water until appropriate volume. Use a flask at least 2 times larger than the media volume.2. For minimal medium, make separate 2X agar and 2X salt flasks. For example, for 1 liter of E plates, make 500 mLs of 2X E salts, and 500 mLs of 2 X agar (1.5 g per 500 mLs). Use at least one flask large enough to mix the media together after autoc......閱讀全文
Pouring Plates
1. For rich media, weigh out appropriate ingredients and place into a flask. Add water until appropriate volume. Use a flask at least 2 times larger
Layered plates
General Information The cells being plated are sandwiched between layers of cell-free agar. The submerged cells grow into smaller colonies and many
Dropout plates for yeast
Materials (Solutions are all available from the media room) 200ml bottle of 2x SD 200ml bottle of 4% agar -- make sure to sign it out 40% g
Preparation of Agar plates
Prepare media and add 1.5 agar before autoclaving it (15g per liter).? After autoclavation, cool the media in a 55 degree waterbath. Do not allow? th
A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the tray
Marcantonio Lab Protocol Manual
Protein GelPreparing and Running a Protein Gel (7% Polyacrylamide)?A. Preparation of Running Gel Solution?1) Add to a 50 ml cylinder:DD-H2O 26 ml30% a
Preparation of Broth and Plates, etc.
Recipes: ? 1)?LB Broth Make 16 gm of LB Broth Base (Gibco #M27800C) up to 800 ml in ddH2O.?Swirl to dissolve, then add 110 μl of 10 N NaOH.??Autoc
Agar Plates for Selection of Clones in Bacteria
Cloning of PCR products Stocks: LB Agar: Luria Broth after Lennox: per Liter Tryptone 10 g Yeast Extract 5 g Sodium Chlori
Marcantonio Lab Protocol Manual——3
Sequencing Gel Preparing and Running a Sequencing Gel (6% Polyacrylamide/Urea)? A. Preparation of Gel Solution? 1) Weigh out 50 g of Urea into a
Preparing Antibiotics Stock Solution and Ampicillin Agar Plates
AMPICILLIN?Beta-lactam-antibiotics are not very stable when dissolved. Slow but steady degradation happens even when frozen to -20?°C. Therefore comme