Chapter 1: Cell Death - Apoptosis and Necrosis
| 1.1 | Introduction | 2 |
| 1.1.1 | Terminology of cell death | 2 |
| 1.1.2 | Differences between necrosis and apoptosis | 3 |
| 1.1.3 | Apoptotic Pathways | 4 |
| 1.2 | Apoptosis Assay Methods | 6 |
| Method/Product selection guide | 7 | |
| 1.2.1 | Methods for studying apoptosis in cell populations | 8 |
| 1.2.1.1 | Assays that measure DNA fragmentation | 8 |
| Apoptotic DNA Ladder Kit | 11 | |
| Cell Death Detection ELISAPLUS | 13 | |
| 1.2.1.2 | Assays that measure apoptosis-induced proteases (caspase) | 16 |
| Caspase 3 Activity Assay | 17 | |
| Anti-PARP | 20 | |
| 1.2.1.3 | Summary of methods for studying apoptosis in cell populations | 22 |
| 1.2.2 | Methods for studying apoptosis in individual cells | 24 |
| 1.2.2.1 | The TUNEL enzymatic labeling assay | 24 |
| In Situ Cell Death Detection Kit, Fluorescein | 27 | |
| In Situ Cell Death Detection Kit, AP | 29 | |
| In Situ Cell Death Detection Kit, POD | 29 | |
| 1.2.2.2 | Assays that measure membrane alterations | 31 |
| Annexin-V-FLUOS | 32 | |
| Annexin-V-FLUOS Staining Kit | 32 | |
| Annexin-V-AlexaTM 568 | 32 | |
| Annexin-V-Biotin | 34 | |
| 1.2.2.3 | Assays that use DNA stains | 36 |
| DAPI, Ethidium bromide, Propidium iodide | 36 | |
| 1.2.2.4 | Summary of methods for studying apoptosis in individual cells | 38 |
| 1.2.3 | Detection of apoptosis-related proteins | 40 |
| Anti-Fas (CD95/Apo-1) | 41 | |
| Anti-Fas-Biotin (CD95/Apo-1) | 42 | |
| Anti-Bcl-2 oncoprotein, human | 44 | |
| Anti-p53 Protein, mutant | 46 | |
| Anti-p53 Protein pan | 46 | |
| Anti-p53 pan | 46 | |
| Anti-p53, Biotin labeled | 46 | |
| Anti-p53, Peroxidase labeled | 46 | |
| p53pan ELISA | 48 |
| 1.3 | Cytotoxicity Assay Methods | 50 |
| 1.3.1 | Relationship between cytotoxicity, apoptosis, and necrosis | 50 |
| 1.3.2 | Methods for studying cytotoxicity | 50 |
| 1.3.2.1 | Assays the measure plasma membrane leakage | 51 |
| Cytotoxicity Detection Kit (LDH) | 52 | |
| Cellular DNA Fragmentation ELISA | 54 | |
| 1.3.2.2 | Assays that measure metabolic activity | 58 |
| Cell Proliferation Kit I (MTT) | 58 | |
| Cell Proliferation Kit II (XTT) | 58 | |
| Cell Proliferation Reagent WST-1 | 58 | |
| 1.3.2.3 | Summary of methods for studying cytotoxicity | 60 |
Chapter 2: Cell Proliferation and Viability
| 2.1 | Introduction | 64 |
| 2.1.1 | Terminology of cell proliferation and viability | 64 |
| 2.1.2 | Cell cycle | 64 |