實驗概要This protocols provides a general protocol for isolation of lymphatic endothelial cells.實驗步驟Dermal Cell Suspensions1. Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery. 2. Dermal sheets were prepared by incubation of split-thickness skin with dispase (50 U/ml) for 30 min at 37°C, and subsequent removal of the epidermis. 3......閱讀全文
Inflammation is a protective response to infection by the immune system that requires communication between different classes of immune cells to coord
EPC Isolation and Characterization1. EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human bloo
Over 1,000 papers and reviews have been written about the role of ceramide in the production of programmed cell death or apoptosis. Ceramide is a sphi
Maeda K, Okubo K, Shimomura I, Funahashi T, Matsuzawa Y, Matsubara K. cDNA cloning and expression of a novel adipose specific collagen-like factor
在免疫學實驗中,比如WB,IHC,IF等,大家都知道選著好正確的一抗,往往實驗就成功了一大半,因此,往往很多研究者便會忽視對二抗,以及其它試劑盒品質的追求。二抗是一種相對而言比較穩定的產品,包裝大,因此很多實驗室都會公用一個二抗。但如果二抗沒有選擇好的話,影響的可能就是一整個實驗室或者課題組了。艾美
比利時魯汶大學Peter Carmeliet院士團隊與中山大學中山眼科中心李旭日教授團隊合作,首次發現了靜息內皮細胞較增殖性內皮細胞具有更高的脂肪酸氧化反應,通過氧化還原穩態上調脂肪酸氧化反應對血管起保護作用。李旭日教授和Peter Carmeliet院士(前排左三, 四)在中山大學中山眼科中心
既往研究顯示,強化血糖控制可以使1型及2型糖尿病患者白蛋白尿、終末期腎病、視網膜病變、心血管疾病、腎病等并發癥風險降低,但并發癥風險的降低,卻難以完全歸因于血糖控制的改善,比如針對DCCT(糖尿病控制和并發癥試驗)研究的分析就發現,僅11%的視網膜病變可通過糖尿病病程及HbA1c進行解釋,余下8
DescriptionThis is a simple assay that can be used in any cell culture lab setup to test the effect of different compounds on endothelial cell migrati
冠狀血管內皮細胞中特異性基因操縱心臟特異性血管中的基因敲除VEGFR2(Kdr)在大多數器官的內皮細胞中普遍表達,過去要在冠狀動脈中特異性靶向該基因幾乎是不可能做到的,因此選擇Kdr基因作為靶基因來驗證CoER-Cre系統在條件性基因敲除中的應用。通過Kdr flox小鼠與CoEC-Cre(Tie2
Endothelial cells respond to treatment with the protease thrombin with increased secretion of the PDGF B-chain. This activation occurs at the transcri
Sphingosine-1-phosphate (S1P) is an example of lipid messengers with both intracellular and extracellular functions. Intracellularly S1P regulates pro
【摘要】 目的:探討堿性成纖維細胞生長因子(bFGF)與膀胱癌發生發展的關系。方法:應用免疫組化方法檢測了48例膀胱癌存檔標本和4例正常膀胱組織中bFGF的蛋白表達。結果:48例膀胱癌存檔蠟塊中有23例陽性,浸潤性癌顯著高于淺表癌(P<0.01),除G1和G2之間外各分級陽性率之間差異也有顯著性。陽
5月15日,中國科學院生物化學與細胞生物學研究所周斌研究組在國際學術期刊Circulation Research上發表了題為“Genetic Targeting of Organ-Specific Blood Vessels”的最新研究成果。該項工作建立一套新的遺傳操作系統以實現更加精確的遺傳靶
5月15日,中國科學院生物化學與細胞生物學研究所周斌研究組在國際學術期刊Circulation Research上發表了題為“Genetic Targeting of Organ-Specific Blood Vessels”的最新研究成果。該項工作建立一套新的遺傳操作系統以實現更加精確的遺傳靶
2013年,美國、德國3位科學家憑借他們所發現的細胞囊泡運輸的調節機制,榮獲2013年諾貝爾生理學或醫學獎。外泌體(exosomes)作為人體內一類重要囊泡,也開始受到越來越多的關注。科學家們已發現,外泌體會參與到免疫應答、凋亡、血管生成、炎癥反應、凝結等重要的生物過程中,細胞會通過分泌外泌體,
4.5.1.1 General informationEndothelial cells possess an endogenous alkaline phosphatase (AP) activity. The enzymatic activity of AP is not restricted
Cell創刊于1976年,現已成為世界自然科學研究領域最著名的期刊之一,并陸續發行了十幾種姊妹刊,在各自專業領域里均占據著舉足輕重的地位。 Cell以發表具有重要意義的原創性科研報告為主,許多生命科學領域最重要的發現都發表在Cell上。本月《Cell》前十名下載論文為: 1. Revi
PriCells: Isolation of endothelial progenitor cells (EPCs) 1. Twenty-four mL venous blood was collected at each time point
下肢缺血試驗應用血管新生是缺血性心血管疾病血流阻塞后改善血流灌注的重要機制。然而,目前臨床上尚缺乏一種簡單、無創和特異的直接評價血管新生的方法。下肢缺血試驗是進行血管新生評價的重要試驗。方法是使用大鼠或小鼠予結扎一側下肢股動脈制備下肢缺血模型,另一側下肢作為對照組。術后使用激光多普勒血流成像儀對雙后
TNFR2 is the receptor for the 171 amino acid 19 kD TNF(beta) (a.k.a. lymphotoxin). TNF(beta) is produced by activated lymphocytes and can be cytotoxic
五、使用儀器發表文章AuthorDateTitleJournalCell TypeCellometer / ApplicationsMahato, Ram INovember 2013Synthesis and Characterization of an Anti-Apoptotic Immu
Adiponectin Replenishment Ameliorates Obesity-Related Hypertension作者:Koji Ohashi; Shinji Kihara; Noriyuki Ouchi; Masahiro Kumada; Koichi Fujita; Aki
Bone marrow contains three types of stem cells:1. Hematopoietic stem cells give rise to the three classes of blood cells that are found in the
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
《Cell Reports》報道,辛辛那提兒童醫院醫學中心和日本橫浜市立大學的科學家們采用新型生物工程手段,開發了一款名為自縮合細胞培養(self-condensation cell culture)技術。這項技術將有助于早日實現用人類自體細胞培養器官/組織的臨床轉化。 “目前,它可以作為治療
隨著最近胰島組織移植的成功,利用組織移植治療器官功能失常的策略正在得到越來越多的關注。而且,在基于干細胞的組織工程學方面的創新進一步顯示出這一策略的巨大醫療潛力。但是,臨床上使用組織移植療法能否成功的關鍵,在于移植到宿主體內的組織能否及時發育出血管網絡并且整合到宿主體內。日前,日本橫濱市立大學(
Isolation of mice glomeruli1. Mice were anesthetized by an intraperitoneal injection of Avertin (2,2,2-tribromoethyl and tertiary amyl alcoho
CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1. Hearts mounted on a Langendorff apparatus were perfused
Mitochondrion-Selective Rhodamines and RosaminesRhodamine 123Rhodamine 123 (R302; FluoroPure Grade, R22420; Figure 12.22) is a cell-permeant, cationic
As tissues grow they require angiogenesis to occur if they are to be supplied with blood vessels and survive. Factors that inhibit angiogenesis might