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1) Determine the number of cells for injection (ie 5′106 ) to determine the number of plates thatwill require trypsinizing (usually a 100% confluent plate of 100mm2 will yield at least 2injections at 5′106 cells/injection)2) Trypsinize the number of plates to be counted all at once3) Collect detached cells in 50 ml conical and spin for 4min at 800 rpm4) Remove sup and resuspend in 25 ml of SFM for counting5) Remove t......閱讀全文
As a primary component of circulatory system, serum is the major reservoir of thousands of proteins secreted or “leaked” from a broad spectrum of